CJCP
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2011 Vol.  13 No.  11
Published: 2011-11-15
CLINICAL RESEARCH
EXPERIMENTAL RESEARCH
CLINICAL EXPERIENCE
CASE REPORT
851 HU Yan, LI Hai-Qi
Assessment and management of rotation and angulation of lower limbs in children Hot!
Most rotational and angulational variations in young children are benign and resolved spontaneously. However, they may cause great concerns to parents. In order to provide a deliberate assessment and management of lower limb rotational and angulational problems, it is necessary for pediatricians to understand the normal variations of lower limb development in healthy children combined with the features of child growth and development and detail physical examinations.                                                                                                                                                                                                                                                     
2011 Vol. 13 (11): 851-854 [Abstract] ( 4692 ) [HTML 1KB] [PDF 385KB] ( 1500 )
873 Gulibaha MAIMAITI, Abulaiti ABDUHAER, XU Pei-Ru
Evaluation of efficacy and safety of loratadine in the treatment of childhood asthma
OBJECTIVE: To evaluate the efficacy and safety of loratadine, a new generation of antihistaminics, in the treatment of childhood asthma. METHODS: The papers related to loratadine treatment for childhood asthma were searched in the database of PubMed, MEDLINE, EMBASE, Cochrance, CNKI and CBMdisc (January 1990 to December 2010) electronically and manually. According to the Cochrane reviewer’s handbook, the quality of the enrolled papers was assessed and a systematic review was performed. RESULTS: A total of 179 papers were obtained. Eleven randomized controlled trials met the criteria and were included in this study. The 11 trials included 317 children with asthma: 159 cases in the loratadine treatment group and 158 cases in the control group. All included studies belonged to the B class according to the quality evaluation criteria. Meta analysis showed that the clinical symptoms were improved more, the forced expiratory volume in 1 second (FEV1) 4 and 8 weeks posttreatment and the peak expiratory flow rate (PEFR) 8 weeks posttreatment were higher in the loratadine treatment group than in the control group. The treatment-related adverse effects, fatigue, tachycardia and palpitation, occurred less in the loratadine treatment group compared with the control group. CONCLUSIONS: Loratadine is safe and effective for the treatment of childhood asthma.
2011 Vol. 13 (11): 873-877 [Abstract] ( 7029 ) [HTML 1KB] [PDF 1248KB] ( 1736 )
CLINICAL RESEARCH
855 WANG Ying-Juan, PAN Kai-Li, ZHAO Xiao-Li, QIANG Huan, CHENG Sheng-Quan
Therapeutic effects of erythropoietin on hypoxic-ischemic encephalopathy in neonates
OBJECTIVE: To study the efficacy of erythropoietinin (EPO) in the treatment of moderate or severe hypoxic-ischemic encephalopathy (HIE) in neonates. METHODS: Seventy neonates with moderate or severe HIE were randomly assigned to two groups: EPO treatment and control (n=35 each). The EPO treatment group included 22 cases of moderate HIE and 13 cases of severe HIE. The control group included 24 cases of moderate HIE and 11 cases of severe HIE. Thirty-five healthy full-term infants served as normal group. The control group received a conventional treatment. Beside the conventional treatment, the EPO treatment group was intravenously injected with EPO of 200 IU/kg?d, 3 times weekly. Routine blood test was performed every 6 days. EPO dose was adjusted based on the results of the routine blood test. The course of EPO treatment was 2 to 4 weeks. Neonatal Behavioral Neurological Assessment (NBNA) was performed at age of 28 days. The infant development test of Child Development Centre of China (CDCC) was performed at ages of 3 months and 6 months. RESULTS: The percentage of normal NBNA scores in the EPO treatment group was significantly higher than that in the control group at age of 28 days (P<0.05), but was significantly lower than that in the normal group (P<0.01). The CDCC test including physical development index (PDI) and physical development index (MDI) showed the percentage of normal results in the EPO treatment group was significantly higher than in the control group at age of 3 months (P<0.05), but was significantly lower than in the normal group (P<0.01). The CDCC test including PDI and MDI showed that the percentage of normal results in the EPO treatment group was significantly higher than in the control group at age of 6 months. The MDI test results in the EPO treatment group were not significantly different from those in the normal group at age of 6 months, but the percentage of normal results in the PDI test in the EPO treatment group was still significantly lower than that in the normal group (P<0.05). CONCLUSIONS: EPO treatment has neuroprotective effects against moderate or severe HIE and improves longterm behavioral neurological developments in neonates.
2011 Vol. 13 (11): 855-858 [Abstract] ( 5337 ) [HTML 1KB] [PDF 926KB] ( 1896 )
859 YANG Wen-Xu, YANG Hui-Ming
Factors influencing the bone mineral density in preterm infants
OBIECTIVE: To study the bone mineral development and the factors influencing the development in preterm infants. METHODS: Ninety preterm and 90 term infants followed up by the child health care service were randomly enrolled. Tibia quantitative ultrasound measurements were used to evaluate bone mineral density described as supersonic speed of sound (SOS) and Z scores at 6 months old (corrected gestational age for preterm infants). The factors influencing bone mineral development were investigated by questionnaire. RESULTS: The SOS values and Z scores in term infants were significantly higher than those in preterm infants at 6 months old. In the preterm group, the SOS values and Z scores were significantly different in infants with different birth weights or gestational ages (P<0.05). The SOS values in preterm infants with different weaning time were significantly different. The Z scores in female preterm infants were significantly higher than that in males (P<0.05). Multiple regression analysis indicated that weaning time and daily time of outdoor activities were independent factors influencing SOS values in preterm infants. CONCLUSIONS: It is helpful to promote bone mineral development by an appropriate weaning time or increasing the time of outdoor activities in preterm infants.
2011 Vol. 13 (11): 859-862 [Abstract] ( 4706 ) [HTML 1KB] [PDF 916KB] ( 1315 )
863 RUAN Min, WANG Ya-Qin, ZHANG Li, LIU Tian-Feng, LIU Fang, LIU Xiao-Ming, ZHANG Jia-Yuan, ZOU Yao, CHEN Yu-Mei, ZHU Xiao-Fan
FLT3 mutations in children with acute myeloid leukemia: a single center study
OBJECTIVE: To study the clinical significance of FMS-like tyrosine kinase 3 (FLT3) mutations including internal tandem duplication (ITD) mutation and point mutation of tyrosine kinase domain (TKD) in children with acute myeloid leukemia (AML). METHODS: Bone marrow samples from 116 children with newly-diagnosed AML were obtained．Gene mutations of FLT3/ITD and FLT3/TKD were detected by RT-PCR. The relationship of FLT3 gene mutations with the clinical characteristics and the therapeutic efficacy was observed. RESULTS: FLT3/ITD and FLT3/TKD mutations were detected in 9 cases (7.8%) and 13 cases (11.2%) respectively out of the 116 children. FLT3/ITD mutations were observed in 3 cases of AML-M3 (3/9; 33.3%) and in 3 cases of AML-M5 (3/9; 33.3%). FLT3/TKD mutations were the most common in AML-M3 patients (10/13; 76.9%). The patients with FLT3/ITD mutations had a significantly higher peripheral WBC count and marrow blast percentage compared with the patients without FLT3/ITD mutations at diagnosis (P<0.01). The 3-year overall survival rate in patients with FLT3/ITD mutations was significantly lower than that in patients without FLT3/ITD mutations (38.9% vs 64.3%; P<0.05).ConclusionsFLT3/TKD mutations are common in children with AML-M3. The AML children with FLT3/ITD mutations present a high peripheral WBC count and a high marrow blast percentage at diagnosis and have an unfavorable outcome.
2011 Vol. 13 (11): 863-866 [Abstract] ( 5811 ) [HTML 1KB] [PDF 971KB] ( 1204 )
867 HAO Jin-Jin, QIU Yi-Ning, ZHOU Dong-Feng, XIAO Yan, LIU Qin, JIN Run-Ming
Comparisons of clinical features of chronic aplastic anemia and myelodysplastic syndrome in children
OBJECTIVE: This study compared the differences in clinical features between chronic aplastic anemia (CAA) and myelodysplastic syndrome (MDS) in children in order to provide a basis for the differential diagnosis of the two diseases. METHODS: A retrospective study of 23 cases of CAA and 9 cases of MDS from September 2007 to September 2010 was performed. The clinical data including routine blood test results, reticulocyte counts, serum lactate dehydrogenase level, serum ferritin level, cytological examination of bone marrow, bone marrow CD34+ cell counts, bone marrow chromosome and FISH test results were compared between the CAA and MDS groups. RESULTS: Neutrophils, reticulocytes, and serum ferritin and lactate dehydrogenase levels increased in the MDS group compared with those in the CAA group. There were significant differences in bone marrow blast cell counts and dyshematopoiesis phenomena of three lines blood cells between the CAA and MDS groups. The bone marrow CD34+ cell counts and the rate of chromosomal abnormalities detected in bone marrow cytogenetic analysis in the MDS group were significantly higher than those in the CAA group. CONCLUSIONS: There are differences in the results of laboratory examinations and morphological and cytogenetic examinations of bone marrow between the children with CAA and MDS. The differences are useful to the differential diagnosis of the two diseases.
2011 Vol. 13 (11): 867-869 [Abstract] ( 4493 ) [HTML 1KB] [PDF 914KB] ( 1100 )
870 HAN Wen, XIE Yong, ZHOU Xin-Long, YIN Li-Ming, REN Shu-Ying
Prognosis of 212 children with asthma: a 5-year follow-up study
OBJECTIVE: To study the prognosis of childhood asthma and the factors influencing asthmatic attacks and prognosis. METHODS: The medical data of 212 children with asthma who were followed up for more than 5 years were retrospectively studied. RESULTS: During the 5-year follow up, asthmatic attacks termination was found in 121 cases (57.1%) and asthma persistence was observed in 91 cases. Respiratory tract infections were found as the major factors inducing asthmatic attacks (71.7%), followed by inhaled allergens (17.0%).The children with asthma induced by respiratory tract infections had a higher remission rate of asthmatic attacks (61.2%) than those induced by allergens (41.7%) or exercises (26.3%). Three risk factors for asthma persistence were identified: concurrent allergic rhinitis and eczema, parental asthma and allergy-induced wheezing. CONCLUSIONS: The 5-year follow-up study demonstrated that asthmatic attacks stopped in the majority of children with asthma. Respiratory tract infections may be the major factors inducing acute asthma attacks. The children with asthma induced by respiratory infections may experience a better outcome. Atopic children or children with the genetic background of atopy are at high risks for the development of persistent asthma.
2011 Vol. 13 (11): 870-872 [Abstract] ( 4736 ) [HTML 1KB] [PDF 911KB] ( 1370 )
878 LI Zhen-Zhen, WANG Zhi-Min, XING Lu, ZHANG Hong, WEN Jian-Guo
Correlation of renal AQP1-4 protein expression with renal parenchyma thickness and glomerular filtration rate in children with congenital hydronephrosis
OBJECTIVE: To study the relationship of renal aquaporin -1, -2, -3, and -4 (AQP1-4) expression with renal parenchymal thickness and glomerular filtration rate (GFR) in children with congenital hydronephrotis. METHODS: Renal tissue samples were obtained from 10 kidneys of 10 children (age: 62.3±18.3 months) with hydronephrosis and who underwent Anderson-Hynes pyeloplasty. Renal control samples were obtained from 6 children (age: 62.7±17.1 months) undergoing nephrectomy for nephroblastoma and were confirmed histologically as normal renal tissues. Renal parenchymal thickness of the hydronephrotic kidneys was measured by ultrasound preoperatively and was verified at operation. Renal GFR was assessed using 99mTc-DTPA scintigraphy preoperatively. Western blot was used to examine the expression of AQP1-4 in the renal tissues. The correlations of renal AQP1-4 expression with the renal parenchymal thickness and GFR were assessed by Pearson correlation analysis. RESULTS: The expression of AQP1-4 in the hydronephrotis group was markedly reduced compared to that in the control group (P<0.05). The mean renal parenchymal thickness of the hydronephrotic kidney was 4.59±2.25 mm measured by ultrasound preoperatively. The mean GFR of the obstructed kidney was significantly lower than that of the contralateral kidney in the hydronephrosis group (40±12 mL/min vs 105±20 mL/min; P<0.05). The expression of AQP1, 2, 3 and 4 was positively correlated with preoperative renal GFR and renal parenchymal thickness in the hydronephrosis group (P<0.05). Renal parenchymal thickness was positively correlated with renal GFR (P<0.05). CONCLUSIONS: The expression of renal AQP1-4 is reduced in children with congenital hydronephrosis. The expression levels of AQP1-4 are positively correlated with renal parenchymal thickness and GFR.
2011 Vol. 13 (11): 878-882 [Abstract] ( 4799 ) [HTML 1KB] [PDF 1006KB] ( 1150 )
883 WU Fan, LI Guo-Lin, SONG Xiao-Hua, SU Hai, CAO Xiao-Chun, LIU Yan-Ling, YE Chun-Feng
Relationship between angiotensin converting enzyme gene polymorphism and essential hypertension in children
OBJECTIVE: To study the relationship between insertion/deletion (I/D) polymorphism of 287 bp in the 16th intron of angiotensin converting enzyme (ACE) and essential hypertension in children. METHODS: I/D polymorphism of 287 bp in the 16th intron of ACE was detected using PCR in 105 children with essential hypertension and 105 normal children as control group. RESULTS: There was an I/D polymorphism in the 16th intron of ACE in the hypertension and the control groups: type II, type ID and type DD. The genotype frequencies of type DD, type ID and type II in the hypertension group were 30.5%, 47.6% and 21.9%, respectively. The genotype frequencies of type DD, type ID and type II in the control group were 14.3%, 46.7% and 39.1%, respectively. There were significant differences in the genotype frequencies of types DD and II between the two groups (P<0.01). The allele frequency of type D (54.3% vs 37.6%) was significantly higher in the hypertension group; in contrast, the allele frequency of type I (45.7% vs 62.4%) was significantly lower than in the control group (P<0.01). CONCLUSIONS: Polymorphism of type II, type ID and type DD exits in ACE. The deletion of 287 bp in the 16th intron of ACE might be associated with the occurrence of essential hypertension in children.
2011 Vol. 13 (11): 883-885 [Abstract] ( 4729 ) [HTML 1KB] [PDF 937KB] ( 1165 )
886 WU Li-Jia, WANG Cheng, LIN Ping, YUAN He-Li, JIN Lou, KANG Mei-Hua, ZHU Li-Ping
Diagnosis of vasovagal syncope in children with postural orthostatic tachycardia syndrome
OBJECTIVE: To study the diagnosis of vasovagal syncope (VVS) in children with postural orthostatic tachycardia syndrome (POTS). METHODS: The clinical data of 57 children with POTS diagnosed by the head-up tilt test between January 2007 and December 2010 were studied retrospectively. Of the 57 children, there were 29 boys and 28 girls who were aged from 5 to 16 years (12.2±1.9 years). RESULTS: Twenty-four (42%) out of 57 children were diagnosed with VVS by prolonging the duration of the head-up tilt test (for the children with orthostatic tolerance): cardioinhibitory (20 cases), mixed (3 cases) and vasoinhibitory (1 case). The POTS children with VVS were older than those without (13.0±1.4 years vs 11.5±2.1 years; P<0.05). There were no significant differences in gender and clinical symptoms between the POTS children with and without VVS. CONCLUSIONS: Some children with POTS may have coexisting VVS, suggesting that it is important to avoid misdiagnosis of VVS by prolonging the duration of head-up tilt test in POTS children with orthostatic tolerance. There are no obvious differences in gender and clinical symptoms between the POTS children with and without VVS.
2011 Vol. 13 (11): 886-888 [Abstract] ( 7080 ) [HTML 1KB] [PDF 909KB] ( 1231 )
889 LI Chun, MA Wei-Ning, WANG Hua
Changes of regulatory T cells in the peripheral blood of children with epilepsy
OBJECTIVE: To study the levels of CD4+ CD25+ Foxp3+ regulatory T cells (Tregs) in the peripheral blood of children with epilepsy and the roles of Tregs in the pathogenesis of epilepsy. METHODS: Forty-one children with epilepsy and thirty-eight healthy children were enrolled. The percentage of CD4+ CD25+ Foxp3+ Tregs in CD4+ T cells and the percentages of CD4+ T cells, CD8+ T cells, nature killer (NK) cells and B cells in lymphocytes were evaluated by flow cytometry. RESULTS: The percentages of peripheral blood CD4+ CD25+ Foxp3+ Tregs and CD4+ T cells and the ratio of CD4+ T cells to CD8+ T cells in epileptic children were (2.4±0.5)%, (35±5)% and 1.32±0.24 respectively, which were significantly lower than those in healthy children ［(6.1±1.2)%, (38±4)% and 1.60±0.24 respectively; P<0.05］. In contrast, the percentages of CD8+ T cells, NK cells and B cells in lymphocytes in epileptic children were significantly higher than those in healthy children［(27±3)% vs (24±3)%, (11.1±5.1)% vs (8.5±1.9)% and (24±9)% vs (16±5)% respectively; P<0.05］. CONCLUSIONS: The abnormal percentage of CD4+ CD25+ Foxp3+ Tregs in the peripheral blood may be involved in the pathogenesis of epilepsy.
2011 Vol. 13 (11): 889-892 [Abstract] ( 4417 ) [HTML 1KB] [PDF 930KB] ( 1149 )
893 ZENG Shuang-Lin, LI Ya-Jun, HUANG Ting, TAN Li-Hua, MEI Xi-Long, SUN Jian-Ning
Relationship between congenital heart disease and bronchial dysplasia
OBJECTIVE: To study the relationship of the incidence of bronchial dysplasia (bronchial anomalous origin and bronchial stenosis) with congenital heart disease. METHODS: A total of 185 children with congenital heart disease or bronchial dysplasia were enrolled. Bronchial dysplasia was identified by the 64-MSCT conventional scanning or thin slice scanning with three-dimensional reconstruction. RESULTS: Forty-five children (25.3%) had coexisting bronchial dysplasia and congenital heart disease. The incidence rate of bronchial dysplasia in children with congenital heart disease associated with ventricular septal defect was higher than in those without ventricular septal defect (33.7% vs 15.0%; P<0.05). There were no significant differences in the incidence rate of bronchial dysplasia between the children with congenital heart disease who had a large vascular malformation and who did not. CONCLUSIONS: Bronchial dysplasia often occurs in children with congenital heart disease. It is necessary to perform a tracheobronchial CT scanning with three-dimensional reconstruction to identify tracheobronchial dysplasia in children with congenital heart disease, especially associated with ventricular septal defect.
2011 Vol. 13 (11): 893-895 [Abstract] ( 4378 ) [HTML 1KB] [PDF 979KB] ( 1045 )
896 YUAN Jin-Na, LIANG Li, CAI Xi-Ding, LI Zhen, BAI Min, GU Cheng-Ping
Impact of gonadotropin-releasing hormone analogs on body mass index in girls with idiopathic central precocious puberty: a long-term follow-up study
OBJECTIVE: To study the impact of gonadotropinreleasing hormone analogs (GnRHa) on body mass index (BMI) in girls with idiopathic central precocious puberty (ICPP). METHODS: One hundred and thirty-four girls with ICPP were enrolled. Fifty-seven out of the 134 girls were treated with GnRHa for 1.69±0.43 years. The height, weight, bone age and BMI were measured before treatment, at the end of the treatment and after reaching near adult height and compared with those in the untreated 77 girls. RESULTS: The adult predicted height standard deviation score (SDS) at the end of treatment was significantly higher than that before treatment (P0.05). With GnRHa treatment, the near-adult height SDS was higher than the target height SDS (P0.05). A significant reduction in the BMI was observed when reaching the near-adult height in the GnRHa treatment group compared to the level of pretreatment and the untreated group (P<0.01). However, the BMI in the GnRHa treatment group before treatment, at the end of the treatment and after reaching near adult height remained in the normal range (±1 SD). CONCLUSIONS: GnRHa may improve the final height in girls with ICPP. The alterations of BMI after GnRHa therapy fluctuate in a normal range.
2011 Vol. 13 (11): 896-899 [Abstract] ( 4756 ) [HTML 1KB] [PDF 970KB] ( 1286 )
900 GUO Ru, MAO Ding-An, LI Jie-Min, LUO Xue-Mei, JIANG Yan, LIU Ji-Hong
Investigation on the behavior problems of children aged 3 to 5 years in Changsha and comparison of the norm of Conners Parent Symptom Questionnaire in Chinese and American urban children
OBJECTIVE: To investigate the behavior problems of children aged 3 to 5 years in Changsha and to compare the differences of the results detected by the norm of Conners Parent Symptom Questionnaire (PSQ) in Chinese and American urban children. METHODS: A total of 854 children aged 3 to 5 years were randomly sampled from 5 districts in Changsha City and their parents completed the Conners PSQ. RESULTS: The assessment by the norm of PSQ in American urban children demonstrated that the average prevalence of behavior problems was 20.4%, with 28.1% for boys and 12.4% for girls. There were significant differences between boys and girls (P<0.01). The factor score of anxiety in girls was markedly higher than that in boys (P<0.01). Learning and psychosomatic problems were the main problems in the children. The prevalences of conduct problems and impulse-restlessness, and abnormal restlessness index detected by the norm of PSQ in Chinese urban children were higher than those detected by the American norm. The prevalences of conduct problems and psychosomatic problems in boys by the norm of PSQ in Chinese urban children were significantly lower than those detected by the American norm, while the total prevalence of behavior problems was higher than that detected by American norm. There was a poor consistency in the assessment results of most factors of the PSQ and the total prevalence of behavior problems detected by the Chinese and American norms (Kappa<0.4). CONCLUSIONS: The prevalence of behavior problems especially learning problems and psychosomatic problems in children aged 3 to 5 years is higher in Changsha. The consistency of assessment results is poor between the norms of China and America. It is recommended to use the China norm of PSQ in Chinese children aged 3 to 5 years because the Chinese norm is in line with China′s national conditions and cultural background.
2011 Vol. 13 (11): 900-903 [Abstract] ( 4967 ) [HTML 1KB] [PDF 916KB] ( 1304 )
904 WEI Hong-Ping, YANG Fang-Ru
Social-psychological factors contributing to male juvenile delinquency
OBJECTIVE: To study the major social-psychological factors contributing to male juvenile delinquency. METHODS: One hundred and thirty-seven cases of male juvenile delinquents (delinquent group) and 145 aged-matched male students (control group) were enrolled in this case-control study. A questionnaire survey was conducted using the Adolescent Self-Rating Life Events Check List, the Coping Style Questionnaire, the Family Environment Scale-Chinese version, and the Social Support Rating Scale. RESULTS: The monovariate analysis showed that the total score and the scores of some factors of negative life events, the scores of immature coping styles and family conflicts, and the proportion of broken families in the delinquent group were significantly higher than those in the control group. In contrast, the scores of educational levels, study stress factor in the negative life events, mature coping styles, family environments and social supports were significantly lower in the delinquent group than those in the control group. The multivariate factors analysis showed that 7 variables were enrolled into the discriminatory equations, including negative life events (interpersonal relationship and healthy adaptation), self-condemn styles, family conflicts, subjective supports, objective supports, and utilization of social supports. The total accuracy of this equation was 92.2%. CONCLUSIONS: Negative life events in the interpersonal relationship and healthy adaptation, self-condemn styles, family conflicts, and weak social support system may be major social-psychological factors contributing to male juvenile delinquency.
2011 Vol. 13 (11): 904-907 [Abstract] ( 6776 ) [HTML 1KB] [PDF 914KB] ( 1159 )
EXPERIMENTAL RESEARCH
908 DENG Xiao-Lu, HE Fang, PENG Jing, YANG Li-Fen, ZHANG Ci-Liu, XIANG Qiu-Lian, WU Li-Wen, WANG Guo-Li, YIN Fei
Influence of lipopolysaccharide on the permeability of rat brain microvascular endothelial cells and the molecular mechanism
OBJECTIVE: To study the influence of lipopolysaccharide (LPS) on the permeability of rat brain microvascular endothelial cells (BMECs) and possible molecular mechanism. METHODS: Monolayers of primary rat BMECs were separated and cultured, and then treated with (LPS group) or without LPS (control group). The barrier integrity was measured by transendothelial electrical resistance (TEER) assay. The degrees of RhoA activation were determined by Pull-down assay. The expression levels of p115RhoGEF, zonula occludens-1 (ZO-1), occludin and claudin-5 proteins were detected by Western blot analysis. RESULTS: The average TEER values of rat BMECs in the LPS group were 108.3±4.2 Ω?cm2 and 85.4±2.5 Ω?cm2 respectively 3 and 12 hrs after LPS treatment, which were significantly lower than that in the control group (159.0±8.6 Ω?cm2). Compared with the control group, the activity of RhoA started to increase 5 minutes after LPS treatment, and the expression of p115RhoGEF protein started to increase 1 hr after LPS treatment and the cellular protein levels of ZO-1, occludin and claudin-5 decreased significantly 3 hrs after LPS treatment in the LPS group (P<0.05). CONCLUSIONS: LPS may activate the p115RhoGEF/RhoA pathway and decrease protein expression of ZO-1, occludin and claudin-5, resulting in an increased permeability of rat BMECs.
2011 Vol. 13 (11): 908-911 [Abstract] ( 5669 ) [HTML 1KB] [PDF 1053KB] ( 1370 )
912 ZHANG Zhi-Min, TIAN Zhao-Fang, LI Yu-Hong, ZHAO Sai
Expression of erythropoietin and its receptor in the brain of newborn rats suffering from fetal distress
OBJECTIVE: To study the expression of erythropoietin (EPO) and its receptor (EPOR) in the brain of newborn rats suffering fetal distress. METHODS: A model of fetal distress was prepared by ligating bilateral uterine arteries of the rats with full-term pregnancy for 10 minutes before cesarean sections. The expression levels of EPO and EPOR in the brain of newborn rats were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot at 0, 2, 6, 12, 24, 48, 72 hrs and 7 days after birth. Serum EPO levels were measured using ELISA simultaneously. The newborn rats born by cesarean sections which were not subjected to uterine artery ligation were used as the control group. RESULTS: The expression of EPO protein and mRNA in brain tissues in the fetal distress group increased significantly compared with the control group 2, 6 and 12 hrs after birth (P<0.05). The expression of EPOR protein and mRNA in brain tissues in the fetal distress group increased significantly compared with the control group 2, 6, 12, 24 and 48 hrs, and 3 days after birth (P<0.05). Serum EPO levels in the fetal distress group were significantly higher than in the control group 2 hrs after birth. CONCLUSIONS: The EPO and EPOR levels in the brain increase quickly after birth in newborn rats suffering from fetal distress. The EPOR is high expressed for a longer time than EPO. This can provide a basis for the treatment of neonatal brain damage induced by fetal distress by exogenous EPO.
2011 Vol. 13 (11): 912-916 [Abstract] ( 4564 ) [HTML 1KB] [PDF 1525KB] ( 981 )
917 PANG Ying, LI Min, ZHANG Jian-Bo, YAO Bin
Effects of Mycobacterium phlei F.U.36 on regulatory T cells and TLR4 expression in asthmatic mice
OBJECTIVE: To study the effects of early intervention on CD4+CD25+ regulatory T cells and TLR4 expression with Mycobacterium phlei F.U.36 in asthmatic mice. METHODS: Thirty female BALB/c mice were randomly divided into three groups: control, asthma model and Mycobacterium phlei F.U.36 treated asthma groups. Asthma was induced by sensitization and challenges with ovalbumin (OVA) in the later two groups. Mycobacterium phlei F.U.36 was intraperitoneally injected 2 weeks before the first sensitization (0.57 μg/time, once every other day for three times) in the intervention group. After 24 hrs of the last challenge, the mice were sacrificed and the left lung tissues were obtained for the observation of lung pathological changes. Splenic mononuclear cells were isolated. The percentage of CD4+CD25+ regulatory T cells in CD4+ T cells and the mean fluorescence intensity of TLR4 on CD4+ CD25+ T cells were detected by flow cytometry. RESULTS: The percentage of CD4+CD25+ regulatory T cells in the asthma model group was significantly lower than that in the control group (P<0.01), but the mean fluorescence intensity of TLR4 on CD4+CD25+ regulatory T cells was not significantly different from the control group. The percentage of CD4+CD25+ regulatory T cells and the mean fluorescence intensity of TLR4 on CD4+CD25+ regulatory T cells increased significantly in asthmatic mice receiving Mycobacterium phlei F.U.36 treatment compared with the asthma group (P<0.01). CONCLUSIONS: Early intervention with Mycobacterium phlei F.U.36 can increase TLR4 expression on CD4+CD25+ cells and the number of CD4+CD25+ regulatory T cells, and thus provides therapeutic effects in asthmatic mice.
2011 Vol. 13 (11): 917-920 [Abstract] ( 4395 ) [HTML 1KB] [PDF 1155KB] ( 897 )
CLINICAL EXPERIENCE
921 FU Qiang, ZHOU Yan-Ling, SONG Xiao-Xiang, WAN Shen-Hong, MAO Li-Ping, HU Jing-Jiang, YU Kong-Jiang, FENG Qi-Hua
Curative effects of low-dose heparin combined with urokinase on primary nephritic syndrome complicated by severe hypercoagulabale state in children
No abstract available
2011 Vol. 13 (11): 921-922 [Abstract] ( 3660 ) [HTML 1KB] [PDF 897KB] ( 859 )
923 CAI Xiao-Tang, LUO Rong, YU Tao, YE Jiu-Cai
Peripheral nerve function in children with hand foot and mouth disease complicated by acute flaccid paralysis of lower limbs
No abstract avaibale
2011 Vol. 13 (11): 923-925 [Abstract] ( 3619 ) [HTML 1KB] [PDF 896KB] ( 1019 )
926 YANG Qing, YOU Ze-Shan
Active immunotherapy for women with unexplained recurrent spontaneous abortion: a follow-up study on growth and development of offspring
No abstract avaibale
2011 Vol. 13 (11): 926-928 [Abstract] ( 3649 ) [HTML 1KB] [PDF 901KB] ( 1157 )
CASE REPORT
929 WANG Li-Bo, MA Hong-Wei, WANG Lin, REN Shuang
Osteopoikilosis complicated by short stature: a case report and a family survey
No abstract available
2011 Vol. 13 (11): 929-930 [Abstract] ( 3209 ) [HTML 1KB] [PDF 996KB] ( 957 )
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