Chinese Journal Of Contemporary Pediatrics

CJCP

	中文版
	English Version

	2013 Vol. 15 No. 3
	Published: 2013-03-15


	OVERSEAS PEDIATRIC RESEARCH CLINICAL RESEARCH EXPERIMENTAL RESEARCH CASE REPORT REVIEW

OVERSEAS PEDIATRIC RESEARCH

	161	Heidi LUDWIG-AUSER, Lauren B. SHERAR, Marta C. ERLANDSON, Adam D. G. BAXTER-JONES, Stefan A. JACKOWSKI, Chris ARNOLD, Koravangattu SANKARAN
		Influence of nutrition provision during the first two weeks of life in premature infants on adolescent body composition and blood pressure ^Hot!
		OBJECTIVE: Adequate nutrition is paramount for premature infants. Longitudinal information is scant on the effects of early nutrition and later growth. The purpose of this study was to determine the influence of early energy and protein provision in premature infants on adolescent body composition and blood pressure. METHODS: In 2007-2008 we obtained data from 36 male (12.3±1.7 years) and 25 female (11.5±1.8 years) adolescents born preterm at <34 weeks gestation (range 23-34 weeks) between October 1st 1989 and December 31st 1995 (birth weight <1850 g). The adolescents were divided into groups depending on infant intake mode (enteral vs parenteral), energy provision (<70 kcal/kg/d and ≥70 kcal/kg/d) and protein provision (>2.5 g/kg/d for ≥5 days and>2.5 g/kg/d for <5 days) during the first 14 days of life. RESULTS: After controlling for birth weight and biological maturity, adolescents who received ≥70 kcal/kg/d during infancy were significantly taller (163±11 cm vs. 156±11 cm) and heavier (58±16 kg vs. 49±16 kg) than adolescents who received <70 kcal/kg/d. There were no significant differences in systolic and diastolic BP and total percent body fat between the two groups. CONCLUSIONS: Our data suggests that higher infant energy provision appears to be related to adolescent size, it does not appear to contribute to adverse risk factors such as higher systolic BP or increased body fat.
		2013 Vol. 15 (3): 161-170 [Abstract] ( 4473 ) [HTML KB] [PDF 897KB] ( 961 )

CLINICAL RESEARCH

	171	YUAN Jie, AN Shu-Hua, GAO Wen-Jie, DU Wen-Jin, SUN Jun-Feng, ZHANG Man, YAO Cong-Zhuo
		Comparative analysis of conventional pulmonary function test results in children with asthma or cough variant asthma
		OBJECTIVE: To compare the conventional pulmonary function test results of children with asthma or cough variant asthma (CVA). METHODS: A total of 140 children, who were diagnosed with asthma or CVA from May 2010 to May 2011, were divided into acute asthma attack (n=50), asthma remission (n=50) and CVA groups (n=40); 30 healthy children were included as a control group. The forced vital capacity (FVC), forced expiratory volume in one second (FEV1), peak expiratory flow (PEF), forced expiratory flow after 25% of vital capacity has been expelled (FEF25), forced expiratory flow after 50% of vital capacity has been expelled (FEF50), forced expiratory flow after 75% of vital capacity has been expelled (FEF75) and maximal midexpiratory flow (MMEF75/25) were measured. RESULTS: The mean percent predicted values of all the above indices were lower than 80% in the acute asthma attack group, with FEF50, FEF75 and MMEF75/25 declining markedly; the mean percent predicted values of FEF75 and MMEF75/25 were lower than 80% in the CVA group. All the pulmonary function indices in the acute asthma attack group were lower than those in the control group. The mean percent predicted values of FVC, FEV1, FEF25 and MMEF75/25 in the asthma remission and CVA groups were lower than in the control group. All the pulmonary function indices in the acute asthma attack group were lower than in the asthma remission and CVA groups, but there were no significant differences between the asthma remission and CVA groups. CONCLUSIONS: There is small and large airway dysfunction, particularly small airway dysfunction, in children with acute asthma attack. Children with CVA present mainly with mild small airway dysfunction, as do those with asthma in remission.
		2013 Vol. 15 (3): 171-174 [Abstract] ( 4838 ) [HTML KB] [PDF 863KB] ( 893 )

	175	ZHU Xiao-Hua, CHEN Qiang, KE Jiang-Wei, LIU Jian-Mei, LI Lan, LI Jian, HE Mei-Juan, HU Ci-Lang
		Clinical analysis of immune function changes in children with bronchial pneumonia
		OBJECTIVE: To investigate changes in serum complement, immunoglobulins and lymphocyte subsets in children with common and severe bronchial pneumonia, and the role of immune function testing in bronchial pneumonia. METHODS: Twenty children with common bronchial pneumonia, 20 with severe bronchial pneumonia and 20 healthy children (as controls) were enrolled in this study. Immunization rate scattering turbidimetry and six-color flow cytometry were used to detect changes in serum levels of IgA, IgG and IgM, complement C3 and C4 and CD3+, CD4+, CD8+, CD16+, CD56+ and CD19+ cells. RESULTS: The IgA levels of children with common and severe pneumonia were significantly lower than in the control group (P<0.05). The IgG level of children with severe pneumonia was significantly lower than in the control group (P<0.05). There were no significant differences in the levels of IgM and complement C3 and C4 between the two pneumonia groups and the control group (P>0.05). Compared with the controls, the children with severe pneumonia showed significantly lower CD4+ and CD3+ counts (P<0.05) and a significantly higher CD19+ count (P<0.05), and the CD16+ and CD56+ counts of children with severe pneumonia were significantly lower than in the controls and in children with common pneumonia (P<0.05). There were no differences in CD8+ count and CD4+/CD8+ ratio between the two pneumonia groups and the control group (P>0.05). CONCLUSIONS: Immune dysfunction exists in children with bronchial pneumonia, especially those with severe pneumonia. Changes in immune function are correlated with the severity of pneumonia. Immune function testing in children with pneumonia has important clinical significance.
		2013 Vol. 15 (3): 175-178 [Abstract] ( 5069 ) [HTML KB] [PDF 866KB] ( 1038 )

	179	XIA Yu, WU Can-Kui, TANG Yin-Yan, CAO Jin
		Differences in the clinical features of Mycoplasma pneumoniae pneumonia among children of different ages
		OBJECTIVE: To investigate the clinical features of Mycoplasma pneumoniae pneumonia (MPP) among children of different ages. METHODS: Retrospective analysis was performed on the clinical data of 112 children who were hospitalized due to MMP between January 2010 and December 2011. The children were divided into 3 groups according to their ages: infants (<3 years; n=20), preschool-aged children (≥3 years; n=41), and school-aged children (6-15.2 years; n=51). The three groups were compared in terms of their clinical symptoms, pulmonary signs, chest X-ray findings and laboratory test results. RESULTS: The infant group presented mainly with expectoration and wheezing, accompanied by low fever. They showed gastrointestinal symptoms as the most common extra-pulmonary manifestation and had evident pulmonary signs. The majority of the school-aged children group presented with high fever and a severe dry cough, and wheezing was seen in several of them. They showed rash as the most common extra-pulmonary manifestation and had slight pulmonary signs. The symptoms of the preschool-aged children group were in between. In the infant and preschool-aged children groups, most showed bronchopneumonia on chest X-ray, while in the school-aged children group, chest X-rays mostly showed segmental parenchymatous infiltration. The infant group had a higher lymphocyte count than the school-aged children group, while the school-aged children group had a higher serum C-reactive protein level than the infant group. CONCLUSIONS: The clinical features of MPP are different among children of different ages, especially between infants and school-aged children.
		2013 Vol. 15 (3): 179-182 [Abstract] ( 4757 ) [HTML KB] [PDF 869KB] ( 1219 )

	183	CHEN Qian, HU Zheng, ZHANG Qi-Hua
		Analysis of Epstein Barr virus infection in 761 hospitalized children
		OBJECTIVE: To study the status of EB virus (EBV) infection and the spectrum of EBV infection-related diseases. METHODS: A total of 761 plasma samples with suspected EBV infection were collected from 761 children (aged from 22 days to 14 years) admitted between August 2010 and July 2011. EBV-DNA of 761 plasma samples was detected by real-time PCR. The epidemiological characteristics and final clinical diagnosis were analyzed based on the clinical data of these EBV-positive hospitalized patients. RESULTS: A total of 109 cases with EBV infection were detected by real-time PCR, with a positive rate of 14.3%. There were significant differences in the positive rate of EBV-DNA among different age groups and between seasons (P<0.05). The positive rate of EBV-DNA in the baby group (<1 year old) was lowest (P<0.05), and the positive rate of EBV-DNA in summer was higher than in winter (P<0.05). The range of plasma EBV-DNA level in children with EBV-DNA positivity was 2.13 to 6.69 (median 3.72). Based on the final diagnosis of 62 EBV-positive hospitalized children, the most common disease was respiratory system infection (39%), such as acute bronchitis, acute upper respiratory infection and bronchopneumonia. CONCLUSIONS: The EBV-DNA positive rate is different among different age groups and between seasons. Respiratory system infection is a leading disease in hospitalized children who are EBV-DNA positive. Real-time PCR assay is useful for rapid and reliable clinical diagnosis of EBV in children.
		2013 Vol. 15 (3): 183-186 [Abstract] ( 4920 ) [HTML KB] [PDF 866KB] ( 1071 )

	187	FENG Jun-Yan, JIA Fei-Yong, JIANG Hui-Yi, LI Ning, LI Hong-Hua, DU Lin
		Effect of infra-low-frequency transcranial magnetic stimulation on motor function in children with spastic cerebral palsy
		OBJECTIVE: To study the therapeutic effects of infra-low-frequency transcranial magnetic stimulation in children with spastic cerebral palsy. METHODS: Seventy-five children with spastic cerebral palsy were randomly divided into two groups: control (n=33) and treatment groups (n=42). The treatment group accepted infra-low-frequency transcranial magnetic stimulation besides conventional comprehensive rehabilitation therapy. The control group only accepted conventional comprehensive rehabilitation therapy. Motor functions were assessed by gross motor function measure (GMFM) and fine motor function measure (FMFM) at one and three months after treatment. RESULTS: Improvement in the ability to sit in the treatment was better than in the control group at one month after treatment (P<0.05). Improvement in the ability to sit, crawl and kneel, total score of GMFM, and improvement of joint active ability of limbs, grasping ability and operating ability in the treatment group were better than the control group at three months after treatment (P<0.05). CONCLUSIONS: Infra-low-frequency transcranial magnetic stimulation can effectively improve motor function in children with spastic cerebral palsy.
		2013 Vol. 15 (3): 187-191 [Abstract] ( 5035 ) [HTML KB] [PDF 876KB] ( 1077 )

	192	LIU Chen-Tao, ZHANG Guo-Yuan, WANG Guo-Li, YIN Fei
		Clinical and electrophysiological characteristics, and prognosis of acute motor axonal neuropathy in children
		OBJECTIVE: To investigate the clinical and electrophysiological characteristics and prognosis of acute motor axonal neuropathy (AMAN) in children in South China. METHODS: The clinical and electrophysiological data of 6 children with AMAN was analyzed, and they were followed up. RESULTS: The mean age of onset was 4.4 years. Most patients came from rural areas and 5 cases had a history of prodromal infection. There were no seasonal differences in clinical onset among the patients. The most common first symptom was muscle weakness, and the mean time from onset to the most severe disease status was 4.2 days. Nerve conduction test results revealed that all patients showed significantly lower amplitude of motor nerve action potential, only 22.3%-73.4% of the lower limit of normal. Injury to the nerves of distal extremities was more serious than injury to the nerves of proximal extremities (P<0.05), while there was no significant difference in the injury to the nerves of upper and lower extremities (P>0.05). Motor nerve conduction velocity and sensory nerve conduction velocity were normal. All patients received intravenous immunoglobulin (IVIG). Of the 6 AMAN patients, 4 could walk independently after a follow-up of 3 months to 1 year. CONCLUSIONS: AMAN in children occurs mostly in rural areas. There is no seasonal difference in the clinical onset of the disease. Muscle weakness is the most common first symptom and the worst status of AMAN appears in the early stage of the disease. Electrophysiological examination provides important information for the diagnosis of AMAN. Some children with AMAN regain the ability to walk independently 1 year after onset. Early application of IVIG treatment may help recovery of neural function.
		2013 Vol. 15 (3): 192-195 [Abstract] ( 4694 ) [HTML KB] [PDF 862KB] ( 952 )

	196	JI Yu-Xiao, ZHANG Hong-Yan, LIN Shu-Xiang
		Single nucleotide polymorphism of FCGR2A gene in Han Chinese children with Kawasaki disease
		OBJECTIVE: To study the association of FCGR2A gene single nucleotide polymorphism (SNP) rs1801274 with Kawasaki disease (KD) susceptibility and the efficacy of intravenous immunoglobulin (IVIG) therapy in Han Chinese children. METHODS: Thirty-five KD children and 25 age-and gender-matched healthy children (control group) were enrolled in the study. Polymerase chain reaction (PCR) and gene sequence analysis were applied to detect SNP of FCGR2A gene rs1801274. These KD patients were classified into two subgroups based on the presence of coronary artery lesion (CAL) following IVIG therapy: CAL (n=13) and non-CAL (n=22). RESULTS: FCGR2A gene SNP rs1801274 was detected in all subjects, including three genotypes (AA, AG and GG). For FCGR2A gene SNP rs1801274, there were significant differences in the genotype and allele frequencies between the KD and control groups (P<0.05), and significant differences in the genotype and allele frequencies were also found between the CAL and non-CAL subgroups (P<0.05). A allele and AA genotype were linked to an increased risk of KD susceptibility (A allele: OR=3.39, 95%CI:1.53-7.50; AA genotype: (OR=4.93, 95%CI:1.61-15.1). Both AG (OR=5.43, 95%CI:1.06-27.8) and G allele (OR=4.88, 95%CI:1.44-16.5) were linked to an increased risk of CAL in KD children. CONCLUSIONS: Polymorphism of the FCGR2A gene SNP rs1801274 is one of the important factors probably influencing susceptibility to KD and efficacy of IVIG therapy on KD in Han Chinese children.
		2013 Vol. 15 (3): 196-200 [Abstract] ( 4601 ) [HTML KB] [PDF 1004KB] ( 811 )

	201	ZHENG Miao-Miao, YUE Li-Jie, CHEN Xiao-Wen, WEN Fei-Qiu, LI Chang-Gang, YANG Chun-Lan, XIE Cai, DING Hui
		Relationship between the methylenetetrahydrofolate reductase gene polymorphism and adverse reactions of high-dose methotrexate in children with acute lymphocytic leukemia
		OBJECTIVE: To study the association between methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and toxicities after high-dose methotrexate (HD-MTX) infusion in children with acute lymphocytic leukemia (ALL). METHODS: MTHFR variants in 52 children with ALL were determined by reverse transcriptase-polymerase chain reaction-denaturing gradient gel electrophoresis and sequencing. Toxicities of children who received HD-MTX chemotherapy were evaluated according to the National Cancer Institute-Common Toxicity Criteria (NCI-CTC). RESULTS: The children carrying MTHFR 1298AC had a higher risk of developing thrombocytopenia compared with the carriers of the 1298 AA genotype (OR=13.7, 95%CI=1.18-159.36, P=0.036). There was no significant difference in HD-MTX chemotherapy-related adverse effects between the patients with different MTHFR C677T or G1793A genotypes. CONCLUSIONS: MTHFR A1298C polymorohism may associate with the toxicity of HD-MTX chemotherapy in children with ALL.
		2013 Vol. 15 (3): 201-206 [Abstract] ( 4859 ) [HTML KB] [PDF 1213KB] ( 963 )

	207	WANG Nian-Rong, HUANG Jian, LI Kai-Ping, ZHAO Yan, WEN Jing, YE Ya, FAN Xin
		Prevalence and risk factors of overweight and obesity among infants in Chongqing urban area
		OBJECTIVE: To investigate the prevalence and risk factors of overweight and obesity among infants in Chongqing urban area, and to provide a basis for early intervention in cases of childhood obesity. METHODS: A total of 2139 infants aged one month were selected by stratified cluster sampling. They underwent growth monitoring and evaluation at 3, 6, 9, 12 and 18 months after birth. Meanwhile, related factors were investigated using a standardized questionnaire. The data was subjected to multiple logistic regression analysis to determine the risk factors for overweight and obesity among infants aged 18 months. RESULTS: The detection rate of overweight and obesity increased rapidly after birth, reaching 26.04% at six months, and then decreased gradually, reaching 15.89% at 18 months. Multiple logistic regression analysis revealed nine variable factors that were significantly correlated with overweight and obesity among infants aged 18 months, including nutritional status of the father, nutritional status of the infants at birth and at 6, 9 and 12 months after birth, feeding patterns at 3 months, frequency of vegetable intake at 12 months, frequency of sweet beverage addition at 18 months, and bedtime at 18 months. CONCLUSIONS: Overweight and obesity are prevalent among infants in Chongqing urban area, and these conditions are affected by multiple factors. Early comprehensive intervention is recommended to curb prevalence.
		2013 Vol. 15 (3): 207-211 [Abstract] ( 3930 ) [HTML KB] [PDF 872KB] ( 782 )

	212	LI Jiu-Jun, ZHANG Tao
		Diagnostic value of serum CRP and procalcitonin levels in children with bloodstream infectionassociated sepsis and septic infection at other sites
		OBJECTIVE: To evaluate the diagnostic value of measuring serum C-reactive protein (CRP) and procalcitonin (PCT) levels, within 6 hours after admission to the pediatric intensive care unit (PICU) in children with bloodstream infection (BSI)-associated sepsis and septic infection at other sites. METHODS: A retrospective analysis was performed on 30 children with a confirmed diagnosis of systemic inflammatory response syndrome who were admitted to the Shengjing Hospital of China Medical University between January 2010 and January 2012. Clinical data on serum CRP, PCT and D-dimer levels were collected within 6 hours after admission. The diagnostic values of the indices were determined by comparative analysis. RESULTS: Serum CRP and PCT levels in children with BSI-associated sepsis were significantly higher than in children with septic infection at other sites (P<0.05), but there was no significant difference in serum D-dimer levels between the two groups (P>0.05). Serum PCT level was superior to serum CRP level in distinguishing children with BSI-associated sepsis from those with septic infection at other sites. Serum PCT level could not realistically be used for diagnosing BSI-associated sepsis when it was less than 2 ng/mL (negative predictive value: 100%), but could be reliably used when it was more than 10 ng/mL (positive predictive value: 77%). CONCLUSIONS: Serum PCT level is superior to serum CRP level in distinguishing children with BSI-associated sepsis from those with septic infection at other sites within 6 hours after admission to the PICU. Serum PCT level has a better diagnostic value for BSI-associated sepsis when it is more than 10 ng/mL.
		2013 Vol. 15 (3): 212-215 [Abstract] ( 4692 ) [HTML KB] [PDF 921KB] ( 1003 )

	216	YUAN Yuan-Hong, XIAO Zheng-Hui, ZHANG Hui, XU Zhi-Yue, LUO Hai-Yan, LU Xiu-Lan
		Changes in T cell subsets in children with sepsis, and their clinical significance
		OBJECTIVE: To observe changes in T cell subsets in children with sepsis and their prognosis, and to investigate the clinical significance of these changes in the occurrence and development of sepsis. METHODS: Fifty children with severe sepsis and 150 children with general sepsis were enrolled as subjects, and 50 age-matched healthy children were included as controls. The percentages of CD3+, CD4+ and CD8+ T cells in peripheral blood and CD4+/CD8+ ratio were measured by flow cytometry. The pediatric critical illness score (PCIS) was calculated within 24 hours of admission. RESULTS: The children with severe sepsis showed significantly lower percentages of CD3+, CD4+ and CD8+ T cells CD4+/CD8+ ratio and PCIS than the controls and children with general sepsis (P<0.01). Among the 200 cases of sepsis, the percentages of CD3+, CD4+ and CD8+ T cells, CD4+/CD8+ ratio and PCIS were significantly lower in the cured group than in the deceased group. CONCLUSIONS: Children with sepsis have different degrees of cellular immunosuppression, and the degree of cellular immunosuppression is significantly correlated with the severity of the disease. Detection of T cell subsets in peripheral blood is of great significance for evaluating immune function and judging disease severity in children with sepsis.
		2013 Vol. 15 (3): 216-218 [Abstract] ( 4695 ) [HTML KB] [PDF 856KB] ( 722 )

	219	LONG Cai-Xia, ZHU Zhi-Yao, XU Zhi-Yue, FAN Jiang-Hua, LIU Mei-Hua
		Clinical risk factors for capillary leak syndrome in children with sepsis
		OBJECTIVE: To investigate the clinical features of capillary leak syndrome (CLS) in children with sepsis, and to analyze its risk factors. METHODS: Clinical data of 384 children with sepsis was studied retrospectively. They included 304 cases of general sepsis, 54 cases of severe sepsis and 26 cases of septic shock, and were divided into non-CLS (n=356) and CLS groups (n=28). Univariate analysis was performed for each of the following variables: sex, age, malnutrition, anemia, coagulation disorders, white blood cell count, C-reactive protein (CRP), procalcitonin (PCT), tumor necrosis factor (TNF), interleukin (IL)-1, IL-6, blood glucose, lactic acid, Pediatric Risk of Mortality (PRISM) III score, pediatric critical illness score (PICS), severe sepsis and number of failed organs≥3. The statistically significant variables (as independent variables) were subjected to multivariate logistic regression analysis. RESULTS: The incidence rate of CLS in children with septic shock, severe sepsis and general sepsis were 42.3%, 20.1% and 1.3%, respectively, with significant differences among them (P<0.01). There were significant differences in anemia, coagulation disorders, CRP, PCT>2 ng/mL, TNF, IL-1, IL-6, blood glucose, lactic acid, PRISM III score, PICS and number of failed organs≥3 between the non-CLS and CLS groups (P<0.05). Severe sepsis/shock and PRISM III score were the independent risk factors for CLS in children with sepsis. CONCLUSIONS: The severity of sepsis and PRISM III score are positively correlated with the incidence of CLS in children with sepsis. Early monitoring of such factors as infection markers and blood glucose in children with severe sepsis and high PRISM III score may contribute to early diagnosis and effective intervention, thus reducing the mortality from CLS in children with sepsis.
		2013 Vol. 15 (3): 219-222 [Abstract] ( 5249 ) [HTML KB] [PDF 870KB] ( 1134 )

EXPERIMENTAL RESEARCH

	223	SHI Rui-Ming, QIANG Hua, ZHANG Yan-Min, MA Ai-Qun, GAO Jie
		Site-directed mutagenesis and protein expression of SCN5A gene associated with congenital Long QT syndrome
		OBJECTIVE: To construct the sodium channel gene SCN5A-delQKP1507-1509 mutation associated with congenital long QT syndrome, and its eukaryotic expression vector, and to examine the expression of mutation protein in human embryonic kidney (HEK) 293 cells. METHODS: Eukaryotic expression vector PEGFP-delQKP-hH1 for SCN5A-delQKP1507-1509 mutation was constructed by rapid site-directed mutagenesis. HEK293 cells were transfected with the wild or mutant vector using lipofectamine, and then subjected to confocal microscopy. The transfected cells were immunostained to visualize intracellular expression of the mutant molecules. RESULTS: Direct sequence and electrophoresis analysis revealed 9 basic group absences at position 1507-1509. The delQKP1507-1509 mutation eukaryotic expression vector was expressed in HEK293 cells. Immunostaining of transfected cells showed the expression of both wild type and mutant molecules on the plasma membrane and there was no difference in the amount of protein, which suggested that the mutant delQKP1507-1509 did not impair normal protein expression in HEK293 cells. CONCLUSIONS: Successful construction of mutant SCN5AdelQKP1507-1509 eukaryotic expression vector and expression of SCN5A protein in HEK293 cells provides a basis for further study on the functional effects of congenital long QT syndrome as a cause of SCN5A mutation.
		2013 Vol. 15 (3): 223-226 [Abstract] ( 4008 ) [HTML KB] [PDF 1285KB] ( 785 )

	227	YANG Yang, KAN Qing, ZHANG Pan, ZHANG Xiao-Qun, ZHOU Xiao-Guang, ZHOU Xiao-Yu
		Bioinformatic analysis of target gene P rediction and related signaling pathways of miRNA-126*
		OBJECTIVE: To bioinformatically predict and analyze target genes of miRNA-126, with the aim of providing certain basis for related research about target genes and regulatory mechanism in the future. METHODS: The miRNA chip technology was applied to measure expression levels of miRNA-126 in 3 time points (embryo 16, 19 and 21 days) of fetal lung development. Then the target genes of miRNA-126* were screened through miRGen2.0 database. Subsequent bioinformatic analysis of these target genes was performed by Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes Pathway analysis (KEGG Pathway analysis). RESULTS: miRNA-126* manifested continuously upregulated expression with the lung development (from embryo 16 to 21 days). There were 422 predicted target genes in total, and the gene set mainly located in glucuronosyltransferase activity, transferase activity (GO molecular function), multicellular organismal development, developmental process (GO biology process) and intracellular part (GO cellular component). The KEGG Pathway analysis demonstrated that the gene set mostly located in RNA degradation (signal transduction pathway) and prion diseases (disease pathway). CONCLUSIONS: The results suggest that miRNA-126* plays a certain role in fetal lung development and provide a basis for lung devlopment research in the future.
		2013 Vol. 15 (3): 227-232 [Abstract] ( 6510 ) [HTML KB] [PDF 902KB] ( 1806 )

CASE REPORT

	233	HAO Xiao-Sheng, CHEN Yin-Bo, HAO Yun-Peng, LIANG Dong
		Paraneoplastic cerebellar degeneration associated with ganglioneuroblastoma in a boy
		No abstract available
		2013 Vol. 15 (3): 233-234 [Abstract] ( 3172 ) [HTML KB] [PDF 830KB] ( 790 )

	235	HAN Dai-Cheng, XIA Shi-Wen
		Concurrence of Marfan syndrome and urinary tract deformities in a neonate
		No abstract available
		2013 Vol. 15 (3): 235-235 [Abstract] ( 3233 ) [HTML KB] [PDF 924KB] ( 577 )

REVIEW

	236	WANG Zheng-Li, YU Jia-Lin
		Recent progress in the diagnosis of neonatal septicemia
		Neonatal septicemia is one of the major causes of morbidity and mortality worldwide during the neonatal period. It can be classified into two subtypes: early-onset sepsis (EOS) and late-onset sepsis (LOS) depending upon the time of onset. In the western developed countries, group B Streptococcal and Escherichia coli are leading pathogens for EOS, while the most frequent microorganism involved in LOS is coagulase negative Staphylococci, which are different from the domestic data. Clinical manifestations of neonatal septicemia are not specific, so that it is often misdiagnosed. This review describes the progress in diagnostic methods for neonatal septicemia, including blood culture, blood cell counts, cytokine profiles and umbilical cord blood examinations. It provides useful information for early diagnosis and treatment of neonatal septicemia.
		2013 Vol. 15 (3): 236-240 [Abstract] ( 5586 ) [HTML KB] [PDF 997KB] ( 1540 )

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