CJCP
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2015 Vol.  17 No.  12
Published: 2015-12-15
CLINICAL RESEARCH
EXPERIMENTAL RESEARCH
CLINICAL EXPERIENCE
CLINICAL RESEARCH
1261 JIAN Shan, WEI Min, HE Yan-Yan, WANG Wei, KANG Yu-Lin, SUN Zhi-Xing
Clinical and genetic analysis of Dent disease in 4 Chinese children Hot!

Objective To study the clinical features and gene mutations of 4 Chinese children with Dent disease. Methods The clinical and laboratory data of 4 children with Dent disease were analyzed retrospectively. Genetic testing of the 4 cases was carried out. Results All of 4 cases were boys. The first impression of Cases 1-3 was Fanconi syndrome. Proteinuria was presented as the first impression in Case 4. All 4 boys presented with low-molecular weight proteinuria (LMWP) and hypercalciuria, including 3 cases with hematuria, 1 case with kidney stones, 2 cases with nephrocalcinosis, 3 cases with hypophosphatemia, and 3 cases with rickets. Mutations of the CLCN5 gene were revealed in three patients (Cases 1, 2 and 4), including exon 6-7del, c.785_787de l(p.263del Leu) and c.1039 C>T (p.Arg347Term). The first two gene mutations had never reported before. Conclusions Urine protein electrophoresis should be carried out for patients with proteinuria. Dent disease should be taken into consideration when patients with Fanconi syndrome have hypercalciuria, nephrocalcinosis or kindey stones. Genetic analyses are needed for a definite diagnosis.
2015 Vol. 17 (12): 1261-1266 [Abstract] ( 4205 ) [HTML 1KB] [PDF 1457KB] ( 987 )
1267 LIU Yang, XU Zhi-Yong, WU Wei-Qing, LUO Fu-Wei, XIE Jian-Sheng
Genetic and prenatal diagnosis for four families with Williams-Beuren syndrome
Williams-Beuren syndrome is a common chromosome microdeletion syndrome. Early diagnosis and treatment are very helpful for patients and their families. This study identified the chromosome karyotype in one fetus with ultrasonography abnormalities and three children with developmental disorders from four families. This provided guidance for subsequent pregnancy and prenatal diagnosis by using routine G-banding chromosome karyotyping analysis, multiplex ligation-dependent probe amplification (MLPA) and array comparative genomic hybridization (arrayCGH). In one amniotic fluid sample from a pregnant woman with fetal abnormalities on an ultrasound screen and three peripheral blood samples from three children with developmental disorders, the decreased signal of ELN gene probes at 7q11.23 and heterozygous deletions at 7q11.23 were detected by MLPA and array-CGH analysis. The laboratory genetic tests of amniotic fluid samples were normal when the mothers from the four families became pregnant again. It was concluded that MLPA and array-CGH are rapid and accurate tools for the diagnosis of Williams-Beuren syndrome and can provide more information for clinical genetic counseling.
2015 Vol. 17 (12): 1267-1272 [Abstract] ( 3736 ) [HTML 1KB] [PDF 3382KB] ( 1068 )
1273 ZHU Li-Na, WANG Yan, PENG Wei, MA Xiu-Wei, YANG Xiao, LIU Xin, FENG Zhi-Chun
Detection of subtelomeric copy number variations in children with intellectual disability
Objective To detect subtelomeric copy number variations in children with genetic intellectual disability (ID) using multiplex ligation-dependent probe amplification (MLPA), and to investigate the pathogenesis of genetic ID. Methods A total of 68 children with ID who had normal results of G-banding karyotype analysis were included in the study. Their subtelomeric copy number variations were detected using MLPA P036. Results Among the 68 children with ID, 7(10%) showed subtelomeric copy number variations, and all the variations were deletion mutations. Among them, 1 case carried 2 subtelomeric microdeletions, and 1 case carried 4 subtelomeric microdeletions. Conclusions Subtelomeric copy number variations are important causes of genetic ID. MLPA can be used as an economic and effective method for investigating the pathogenesis of genetic ID.
2015 Vol. 17 (12): 1273-1276 [Abstract] ( 3251 ) [HTML 1KB] [PDF 1013KB] ( 778 )
1277 LIU Chao, LU Bin, ZHANG Yu, XU Chuan-Guo, PENG Feng-Xiang, MA Li-Fang, LIU Li-Li, NIE Wen-Ying
Clinical efficacy of structured institution-based teaching programme combined with family rehabilitation training in treatment of childhood autism
Objective To investigate the clinical efficacy of a structured institution-based teaching programme combined with family rehabilitation training in the treatment of childhood autism. Methods One hundred children with autism were divided into a combination therapy group (n=50) and a control group (n=50). The children in the control group received a structured institution-based teaching programme, and the children in the combination therapy group received a family rehabilitation training besides the structured institution-based teaching programme. Comparisons were made between the two groups by the Autism Behavior Checklist (ABC) score, Autism Treatment Evaluation Checklist (ATEC) score, and Chinese version of Psychoeducational Profile (C-PEP) sore. Results After 12-months training, each dimension score and total score of ABC in the combination therapy group were all significantly lower than those in the control group (P<0.05). The combination therapy group had significantly lower dimension scores and total score of ATEC than the control group (P<0.05). Each dimension score and total score of C-PEP were significantly higher in the combination therapy than in the control group (P<0.05). Conclusions As an effective treatment mode for childhood autism, structured institution-based teaching programme combined with family rehabilitation training is worthy of clinical promotion and application.
2015 Vol. 17 (12): 1277-1281 [Abstract] ( 4139 ) [HTML 1KB] [PDF 1110KB] ( 1128 )
1282 ZHOU Hao, ZHANG Li-Li, YAN Wei-Li, XU Xiu, WANG Yi
Construct validity of the Autism Spectrum Rating Scale of the Revised Chinese Version
Objective To investigate the construct validity of the Autism Spectrum Rating Scale of Revised Chinese Version (RC-ASRS). Methods Seven hundred and one children aged 6-12 years old were recruited from one primary school in the Minhang District of Shanghai. The parents of the children completed the RC-ASRS questionnaire. Mpuls 6.0 Software was used to conduct the construct validity analysis. Results A total of 671 questionnaires (95.7%) were retrieved, involving 368 boys (54.8%) and 303 girls (45.2%). The 3 factor structure of the RC-ASRS had better model fitting indices, 0.051 for root mean square error of approximation (RMSEA), 0.889 for comparative fit index (CFI) and 0.884 for Tucker-Lewis index (TLI), compared with the original ASRS, 0.060 for RMSEA, 0.829 for CFI and 0.823 for TLI. Conclusions The RC-ASRS may serve as a reliable and valid tool for screening autistic symptoms in China.
2015 Vol. 17 (12): 1282-1285 [Abstract] ( 4649 ) [HTML 1KB] [PDF 1062KB] ( 1020 )
1286 DU Xia, LI Yu-Ling, ZHANG Yu-Zhu, DONG He-Yu, DING Y
Psychological and behavioral problems and related influencing factors in school-age twins
Objective To investigate the influencing factors for psychological and behavioral problems in schoolage twins and to provide a basis for reducing the incidence of these problems. Methods The psychological and behavioral problems were assessed in 147 pairs of twins aged 6-12 years in Huhhot, China, using the Achenbach Child Behavior Checklist (CBCL). The Family Adaptability and Cohesion Evaluation Scales and Family General Condition Questionnaire were used to collect data. Results The overall detection rate of psychological and behavioral problems in these children was 24.1%; the detection rate of thought problems (15.3%) was the highest, and the detection rate of physical discomforts (3.4%) was the lowest. According to the multiple logistic regression analysis, full-term birth or not was significantly associated with withdrawn behavior and total score on the CBCL (P<0.05); temperament type was significantly associated with thought problems and overall detection rate of psychological and behavioral problems (P<0.05); father's child-bearing age was significantly associated with thought problems (P<0.05); family cohesion was significantly associated with discipline violation (P<0.05); family adaptability was significantly associated with thought problems (P<0.05); family rearing patterns were significantly associated with socialization problems (P<0.05). Conclusions Children's temperament type, full-term birth or not, father's child-bearing age, family cohesion, family adaptability, and family rearing patterns are the main influencing factors for the psychological and behavioral problems in school-age twins.
2015 Vol. 17 (12): 1286-1291 [Abstract] ( 3612 ) [HTML 1KB] [PDF 1291KB] ( 951 )
1292 DAI Bao-Quan, YUAN Xun-Tao, LIU Jin-Ming
Value of serum procalcitonin for the guidance of antibiotic therapy in children with lower respiratory tract infection
Objective To evaluate the value of serum procalcitonin (PCT) for the guidance of antibiotic therapy in children with lower respiratory tract infection (LRTI). Methods A prospective randomized controlled study was conducted in 396 children with LRTI who visited Weifang Maternity and Child Care Hospital. The participants were randomly assigned into a PCT group in which the antibiotic therapy was guided by serum PCT level and a control group in which the standard therapy was given according to clinical guidance. Afterwards, a subgroup analysis was performed according to whether the patient was diagnosed with community-acquired pneumonia (CAP). After 14-day treatment, antibiotic prescription rate, duration of antibiotic treatment, and side events were compared between the groups. Results A total of 396 cases were recruited and equally assigned into the PCT group and the control group, among whom the numbers of the children with CAP were 125 and 123, respectively. The mean duration of antibiotic treatment was significantly shorter in the PCT group than in the control group (P<0.05). The subgroup analysis showed that the duration of antibiotic treatment in both CAP and non-CAP PCT subgroups was significantly shorter than in the control subgroups (P<0.05), however, the antibiotic prescription rate in the non-CAP PCT subgroup was significantly higher than that in the non-CAP control subgroup (P<0.05). There were no differences in the rate and duration of side events from antibiotic therapy, hospitalization rate, the length of hospital stay, and safety between the PCT and control groups. Conclusions Serum PCT-based guidelines on antibiotic use can shorten the duration of antibiotic therapy in children with LRTI.
2015 Vol. 17 (12): 1292-1296 [Abstract] ( 5484 ) [HTML 1KB] [PDF 1162KB] ( 1428 )
1297 YAN Hua-Jie, HE Dong-Ping, SHENG Jun, DONG Wei, LIU Jia, SHAO Jie
Association between parainfluenza virus infection and climatic factors in children
Objective To study the association between acute respiratory human parainfluenza virus (HPIV) infection and climatic factors in children. Methods A total of 2526 throat swab samples were collected from children with acute respiratory infection who visited the Pediatric Clinic of Shanghai Jiading Nanxiang Hospital between 2011 and 2013. HPIV was detected by multiplex RT-PCR. Related meteorological data were collected, including monthly mean temperature, monthly mean humidity, and monthly total rainfall. The association between HPIV detection rate and climatic factors was analyzed by Spearman's or Pearson test. Results During the three years, the overall HPIV detection rate was 5.62% (142/2526), and HPIV-1 was the most common type (46.5%), followed by HPIV-3(31.0%), HPIV-2(17.6%), and HPIV-4(4.9%). There were significant differences in the detection rates of HPIV-1 and HPIV-2 in different seasons, and the detection rates of both were the highest in summer (P<0.05). HPIV positive rate was positively correlated with monthly mean temperature (r=0.598; P<0.01) and monthly total rainfall (rs=0.602; P<0.01). Conclusions The activity of HPIV in children is correlated with climatic factors, particularly temperature and rainfall.
2015 Vol. 17 (12): 1297-1300 [Abstract] ( 4261 ) [HTML 1KB] [PDF 1280KB] ( 740 )
1301 JIANG Kun, LU Xiao-Xia, WANG Ying, CHEN He-Bin, SHU Lin-Hua
Relationship between serum 25-hydroxyl-vitamin D3 levels and galectin-3 levels in serum and bronchoalveolar lavage fluid in children with asthma
Objective To investigate the changes of the levels of galectin-3(Gal-3) in serum and bronchoalveolar lavage fluid (BALF) of children with asthma whose have different serum levels of 25-hydroxyl-vitamin D3[25(OH)D3]. Methods Fifty children with asthma between January 2013 and December 2014 were enrolled as the asthma group, and they were classified into 25(OH)D3 sufficient (n=7), insufficient (n=12) and deficient subgroups (n=31) according to the serum levels of 25(OH)D3. Twenty children with abnormal airway or tracheal foreign bodies served as the control group. The levels of 25(OH)D3, Gal-3 and total IgE in serum and Gal-3 levels in BALF were measured using ELISA. Results The serum levels of 25(OH)D3 in the asthma group were lower than in the control group (P<0.05). The 25(OH)D3 deficient subgroup displayed the highest percentages of neutrophils, eosinophils and epithelial cells in BALF, followed by the 25(OH)D3 insufficient subgroup and the 25(OH)D3 sufficient subgroup (P<0.05). The percentages of neutrophils, eosinophils and epithelial cells in BALF in the three subgroups were all higher than in the control group (P<0.05). In children with asthma, serum levels of 25(OH)D3 were negatively correlated with the percentages of neutrophils, eosinophils and epithelial cells in BALF (r=-0.683, -0.795 and -0.670 respectively; P<0.05); and a negative correlation was also seen between serum 25(OH)D3 levels and serum Gal-3 and total IgE levels (r=-0.759 and -0.875 respectively; P<0.05). Conclusions The children with asthma have low serum levels of 25(OH) D3. 25(OH) D3 and Gal-3 may be involved in the airway inflammation and the development of asthma.
2015 Vol. 17 (12): 1301-1305 [Abstract] ( 3883 ) [HTML 1KB] [PDF 1295KB] ( 789 )
1306 WANG Jian-Rong, FENG Rui-Hua, Duolikun MUZHAPAER
Correlations of IL-4R gene polymorphism and serum IgE levels with asthma predictive index
Objective To study the correlations of IL-4R gene polymorphism and serum IgE levels with asthma predictive index (API) in children. Methods One hundred and sixty-seven children with positive API, 187 children with negative API and 203 healthy children (control group) were enrolled. PCR and DNA sequencing were used to identify genotypes of the Arg551Gln locus in IL-4R gene. Serum IgE levels were measured using ELISA. Results There was no significant difference in the genotype frequencies of the Arg551Gln locus in IL-4R gene among the positive API, negative API and control groups. Serum IgE levels in the positive API group were significantly higher than in the negative API and control groups (P<0.01). In the positive API group, the children aged less than 2 years had significantly lower serum IgE levels than those aged over 2 years (P<0.01). Conclusions There is no correlation between the Arg551Gln polymorphism in IL-4R gene and API results. API positivity is correlated with elevated serum IgE levels. An older age (>2 years) may be a risk factor for increased serum IgE levels in children with positive API.
2015 Vol. 17 (12): 1306-1310 [Abstract] ( 3761 ) [HTML 1KB] [PDF 1551KB] ( 657 )
1311 ZHAO Feng-Xia, LIU Guang-Hui, ZHANG Jian
Value of IL-6 and IL-8 in the diagnosis of neonatal sepsis
Objective To explore the significance of interleukin-6(IL-6) and IL-8 in the diagnosis of neonatal sepsis. Methods This was a prospective study conducted between August 2014 and February 2015. A total of 140 neonates who were suspected infectious were enrolled and classified into a sepsis group (n=49) and a local infection group (n=91). Sixty-one neonates who were non-infectious served as the control group. Serum levels of IL-6 and IL-8 were measured before treatment and 3 days after treatment. The value of serum IL-6 and IL-8 for the diagnosis of neonatal sepsis was assessed by receiver operating characteristic (ROC) curve analysis. Results Before treatment, serum levels of IL-6 and IL-8 in the sepsis group were higher than those in the local infection and control groups (P<0.05), and the local infection group had higher serum levels of IL-6 and IL-8 than the control group (P<0.05). After three days of treatment, the serum IL-6 level in the sepsis group remained higher than that in the local infection and control groups (P<0.05), and the local infection group had higher serum level of IL-6 than the control group (P<0.05). There was no significant difference in the serum IL-8 level among the three groups. According to the ROC curve, when the cut-off value of serum IL-6 was 32 pg/mL, the sensitivity, specificity and accuracy of serum IL-6 for the diagnosis of neonatal sepsis were 87.8%, 79.6% and 81.6% respectively; when the cut-off value of serum IL-8 was 54 pg/mL, the sensitivity, specificity and accuracy of serum IL-6 for the diagnosis of neonatal sepsis were 77.6%, 63.8% and 67.2% respectively. With the combination of serum IL-6 and IL-8 levels, the sensitivity, specificity and accuracy for the diagnosis of neonatal sepsis were 71.4%, 86.2% and 82.6% respectively. Conclusions IL-6 and IL-8 participate in the inflammatory response and the serum levels of both vary with the severity of infection. The diagnostic value of IL-6 for neonatal sepsis is higher than IL-8. The combined detection of serum levels IL-6 and IL-8 may increase the accuracy of diagnosis of neonatal sepsis.
2015 Vol. 17 (12): 1311-1315 [Abstract] ( 4497 ) [HTML 1KB] [PDF 1352KB] ( 1161 )
1316 WANG Xiao-Lei, ZHANG Le, LI Ya-Wen, HOU Hong-Mei, SUN Hai-Bin
Association between toll-like receptors 2 and 5 polymorphisms and neonatal sepsis
Objective To study the association between single nucleotide polymorphisms(SNP) in toll-like receptors (TLR) 2 and 5 genes and the susceptibility to neonatal sepsis. Methods One hundred and fourteen newborn infants who were diagnosed with clinical sepsis (case group) between May 2011 and January 2014 and 172 newborn infants without infection(control group) were enrolled in this study. The polymorphisms of TLR2(rs5743708 and rs3804099) and TLR5(rs5744105) were analyzed using a SNaPshot multiplex reaction to compare the genotypic and allelic frequencies between two groups. The relationship between TLR genotypes and susceptibility to sepsis was analyzed by logistic regression models. Results Significant differences in genotypic frequencies of TLR2 rs3804099(C/T) and TLR5 rs5744105(C/G) were found between the two groups (P<0.05), but there was no significant difference in allelic frequencies of all the SNPs above between the two groups (P>0.05). The genotype on TLR2 rs5743708 was GG and no mutation was found in both groups. In regression models, birth weight (OR=3.065; P<0.05) and gestational age (OR=3.301; P<0.05) were closely associated with neonatal sepsis. Sex (OR=1.107, P>0.05), polymorphisms in rs3804099(OR=0.876; P>0.05) and polymorphisms in rs5744105(OR=0.820; P>0.05) genes were not risk factors for neonatal sepsis. Conclusions TLR2 and 5 polymorphisms (rs5743708, rs3804099 and rs5744105) may not serve as the susceptible gene for sepsis in newborn infants.
2015 Vol. 17 (12): 1316-1321 [Abstract] ( 3854 ) [HTML 1KB] [PDF 1380KB] ( 865 )
1322 CHEN Nan, WEN Xiao-Hong, HUANG Jin-Hua, WANG Shui-Yun, ZHU Yue-E
Predictive value of qualitative assessment of general movements for adverse outcomes at 24 months of age in infants with asphyxia
Objective To investigate the predictive value of the qualitative assessment of general movements (GMs) for adverse outcomes at 24 months of age in full-term infants with asphyxia. Methods A total of 114 fullterm asphyxiated infants, who were admitted to the neonatal intensive care unit between 2009 and 2012 and took part in follow-ups after discharge were included in the study. All of them received the qualitative assessment of GMs within 3 months after birth. The development quotient was determined with the Bayley Scales of Infant Development at 24 months of age. Results The results of the qualitative assessment of GMs within 3 months after birth showed that among 114 infants, 20(17.5%) had poor repertoire movements and 7(6.1%) had cramped-synchronized movements during the writhing movements period; 8 infants (7.0%) had the absence of fidgety movements during the fidgety movements period. The results of development quotient at 24 months of age showed that 7 infants (6.1%) had adverse developmental outcomes:6 cases of cerebral palsy and mental retardation and 1 case of mental retardation. There was a poor consistency between poor repertoire movements during the writhing movements period and the developmental outcomes at 24 months of age (Kappa=-0.019; P>0.05). There was a high consistency between cramped-synchronized movements during the writhing movements period and the developmental outcomes at 24 months of age (Kappa=0.848; P<0.05), and the results of predictive values of cramped-synchronized movements were shown as follows:predictive validity 98.2%, sensitivity 85.7%, specificity 99.1%, positive predictive value 85.7%, and negative predictive value 99.1%. There was a high consistency between the absence of fidgety movements during the fidgety movements period and the developmental outcomes at 24 months of age (Kappa=0.786; P<0.05), and its predictive values were expressed as follows:predictive validity 97.4%, sensitivity 85.7%, specificity 98.1%, positive predictive value 75.0%, and negative predictive value 99.1%. Conclusions Cramped-synchronized movements and absence of fidgety movements can predict adverse developmental outcomes at 24 months of age in full-term infants with asphyxia.
2015 Vol. 17 (12): 1322-1326 [Abstract] ( 4224 ) [HTML 1KB] [PDF 1286KB] ( 904 )
1327 FENG Zong-Tai, YANG Zu-Ming, GU Dan-Feng, YANG Xiao-Lu
Clinical efficacy of heated humidified high-flow nasal cannula in preventing extubation failure in neonates:a Meta analysis
Objective To evaluate the efficacy and safety of heated humidified high-flow nasal cannula (HHHFNC) in preventing extubation failure in neonates. Methods A literature search was performed using PubMed, Cochrane Library, FMRS, and CNKI to collect the randomized controlled trials (RCTs) and quasi-RCTs which compared the clinical efficacy of HHHFNC and nasal continuous positive airway pressure (NCPAP) in preventing extubation failure in neonates. The identified studies were finally selected after full-text search and quality assessment and then subjected to a Meta analysis using RevMan 5.3. Results Five eligible trials involving 1040 neonates were included in the Meta analysis. The Meta analysis showed that there was no significant difference in treatment failure rate between the HHHFNC and the NCPAP groups. The HHHFNC group had significantly lower incidence rates of nasal trauma (OR=0.49, 95% CI:0.34-0.71, P=0.0001) and pneumothorax (OR=0.27, 95% CI:0.07-0.97, P=0.04) than the NCPAP group, but there were no significant differences in the duration to reach full oral feedings and the incidence rates of serious adverse events or other complications between the two groups, such as in-hospital mortality, bronchopulmonary dysplasia, intraventricular hemorrhage, necrotizing enterocolitis, and retinopathy of prematurity. Conclusions HHHFNC is safe and effective in preventing extubation failure in neonates.
2015 Vol. 17 (12): 1327-1332 [Abstract] ( 4411 ) [HTML 1KB] [PDF 1485KB] ( 1204 )
1333 YANG Xiao-Yan, HU Yan-Ling, CHEN Chao, TANG Jun, ZHAO Jing, MU De-Zhi
A pilot study on bacterial culture results of expressed breast milk
Objective To study the bacterial culture results of expressed breast milk. Methods A total of 1178 expressed breast milk samples were collected for bacterial culture. The breast milk sampled from the mothers of preterm neonates (n=615) and term neonates (n=563) who were hospitalized between May 2014 and April 2015. Results There was no significant difference in bacterial counts between the preterm and term sample groups (P>0.05). Potential intestinal pathogens were found in 55 samples (4.63%) of the 1178 samples, with no significant difference between the preterm and term sample groups (P>0.05). The second expressed milk samples from 33 mothers were cultured. Only 10 samples (30%) were found to have the same bacteria as the first time. The detection rate of bacterial load of ≥ 105 CFU/mL was higher in those samples with potential intestinal pathogens, as compared with those samples without potential intestinal pathogens (43.64% vs 14.87%; P<0.05). There was no correlation between the incidence of neonatal infections and potential intestinal pathogens in breast milk. Conclusions Breast milk is not sterile. Bacterial loads and phylotypes are variable. Random breast milk cultures can neither describe bacterial colonies in breast milk, nor be a predictor of neonatal infection.
2015 Vol. 17 (12): 1333-1337 [Abstract] ( 3402 ) [HTML 1KB] [PDF 1328KB] ( 693 )
1338 LI Su-Yun, QIAN Xu-Guang, ZHAO Yi-Li, FU Wen-Jie, TAN Xiao-Ru, LIU Zhen-Huan
Risk factors for interictal epileptiform discharges on electroencephalogram in children with spastic hemiplegic cerebral palsy
Objective To investigate the clinical symptoms and features of interictal epileptiform discharges (IED) on electroencephalogram (EEG) in children with spastic hemiplegic cerebral palsy (CP) and to analyze the risk factors for IED. Methods Eighty-three children with spastic hemiplegic CP were recruited, and their clinical data, results of video-electroencephalogram, imaging findings, and cognitive levels were collected. The influencing factors for IED were determined by multiple logistic regression analysis. Results The incidence of epilepsy was 13% in children with spastic hemiplegic CP; 34% of these cases had IED. The incidence of epilepsy in children with IED (32%) was significantly higher than that in those without IED (4%) (P<0.01). The incidence of IED in children with complications and brain cortex impairment increased significantly (P<0.01). The incidence of IED varied significantly between patients with different cognitive levels (P<0.01). Brain cortex impairment (OR=11.521) and low cognitive level (OR=2.238) were risk factors for IED in children with spastic hemiplegic CP (P<0.05). Conclusions Spastic hemiplegic CP is often found with IED on EEG, and the incidence of epilepsy is higher in children with IED than in those without IED. Brain cortex impairment and low cognitive level have predictive values for IED in children with spastic hemiplegic CP.
2015 Vol. 17 (12): 1338-1341 [Abstract] ( 3458 ) [HTML 1KB] [PDF 1276KB] ( 624 )
EXPERIMENTAL RESEARCH
1342 PENG Li, HE Qing-Nan, LI Xiao-Yan, SHUAI Lan-Jun, CHEN Hai-Xia, LI Yong-Zhen, YI Zhu-Wen
Effect of Homeobox A13 transfection on epithelial-mesenchymal transition and bone morphogenetic protein-7 expression in kidney tubular epithelial cells
Objective To examine the transfection of Homeobox A13(HOXA13) on epithelial-mesenchymal transition (EMT) and the expression of bone morphogenetic protein-7(BMP-7) induced by albumin-overload in human kidney tubular epithelial cells (HKCs). Methods The cultured HKCs were treated with 20 mg/mL human serum albumin (HSA) for 48 hours. Protein expression of cytokeratin (CK), vimentin and HOXA13 in the HKCs was assessed by Western blot. Protein expression of CK, vimentin, and BMP-7 was also detected in HKCs transfected with lipofectamine contained HOXA13 DNA. Results HSA induced EMT in HKCs, presented by decreased CK expression (P<0.01) and increased vimentin expression (P<0.01). The up-regulated expression of HOXA13 transfected by lipofectamine inhibited the level of EMT induced by HSA in HKCs (P<0.05). The decreased rate of BMP-7 protein expression induced by HSA was inhibited by over-expressed HOXA13 in HKCs (P<0.05). Conclusions Transfection of HOXA13 in HKCs could inhibit the degree of EMT induced by albumin-overload, possibly by increasing BMP-7 expression.
2015 Vol. 17 (12): 1342-1347 [Abstract] ( 3098 ) [HTML 1KB] [PDF 2109KB] ( 689 )
1348 ZHAO Feng-Yan, TANG Jun, ZHANG Li, LI Shi-Ping, FENG Yi, LIU Hai-Ting, QU Yi, MU De-Zhi
Role of long non-coding RNA BC088414 in hypoxic-ischemic injury of neural cells
Objective To investigate the role of long non-coding RNA (lncRNA) BC088414 in hypoxic-ischemic injury of neural cells. Methods Rat adrenal pheochromocytoma (PC12) cells were divided into four groups:normoxic, oxygen glucose deprivation (OGD), siRNA-normoxic (siRNA group) and siRNA-OGD (n=3 each). Cells were incubated in glucose-free and serum-free DMEM medium under the conditions of 37℃ and 1% O2+99% N2/CO2 for 6 hours to establish an in vitro hypoxic-ischemic model. Quantitative real-time PCR was used to measure mRNA expression of lncRNA BC088414, β2-adrenoceptor (Adrb2), and caspase-6(CASP6). siRNAs were used to inhibit BC088414 expression in PC12 cells. The TUNEL method was used to measure cell apoptosis. Results The OGD group had a significantly higher cell apoptotic index than the normoxic group (P<0.01). After inhibition of BC088414 expression, the OGD group had a significantly reduced apoptotic index (P<0.05). The OGD group had significantly higher mRNA expression levels of lncRNA BC088414, Adrb2, and CASP6 compared with the normoxic group (P<0.05). The siRNA -normoxic group had significantly lower mRNA expression levels of Adrb2 and CASP6 than the normoxic group (P<0.05), and the siRNA-OGD group also had significantly lower mRNA expression levels of Adrb2 and CASP6 than the OGD group (P<0.05). Conclusions LncRNA BC088414 may promote apoptosis through Adrb2 and CASP6 and aggravate neural cell injury induced by hypoxia-ischemia.
2015 Vol. 17 (12): 1348-1353 [Abstract] ( 3280 ) [HTML 1KB] [PDF 1619KB] ( 732 )
1354 YANG Li-Fen, KONG Hui-Min, ZHANG Xiao-Qing, YIN Fei
Roles of PKCα on the biological functions of T cells
Objective To study the roles of PKCα on the proliferation, apoptosis, differentiation, cytokine production and inducible regulatory T cell (iTreg) induction of T cells. Methods T cells from WT (PKCα+/+) or PKCα knockout (PKCα-/-) mice were isolated and cultured in vitro. T cell proliferation and apoptosis were determined using 3H thymidine incorporation and CSFE/Annexin V staining. Cytokines production (IL-2, IL-4, IFN-γ and IL-17) was detected using ELISA. CD4+T cells were isolated and cultured in vitro via Th17 or iTreg biased condition. Flow cytometry was used to detect the cell differentiation. Results The production of IL-2 upon TCR stimulation increased, while the contents of IL-4 and IL-17 decreased in the PKCα-/- group compared with the PKCα+/+ group. The differentiation rate of Th17 cells decreased, while the iTreg production increased in the PKCα-/- group compared with the PKCα+/+ group. Conclusions PKC-α is proinflammatory.
2015 Vol. 17 (12): 1354-1359 [Abstract] ( 3771 ) [HTML 1KB] [PDF 1766KB] ( 735 )
CLINICAL EXPERIENCE
1360 HAO Xiao-Sheng, WANG Cui-Ying, CHEN Yin-Bo
Hemophagocytic lymphohistiocytosis manifesting as sepsis in children:a report of 6 cases
No abstract available
2015 Vol. 17 (12): 1360-1362 [Abstract] ( 3123 ) [HTML 1KB] [PDF 1090KB] ( 755 )
1363 CHEN Chang-Bin, YOU Jie-Yu, ZHANG Wen-Ting
Misdiagnosis of childhood eosinophilic gastroenteritis:an analysis of 12 cases
No abstract available
2015 Vol. 17 (12): 1363-1365 [Abstract] ( 2801 ) [HTML 1KB] [PDF 1086KB] ( 690 )
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