Levels of IL24 , IL210 and IFN2γ in rats with asthma
LI Guang, ZIAO Yu-Ying, QU Shu-Qiang, LU Xiao-Hui, LIU Chen, TANG Jian-Min, WANG Li-Qun, ZHANG Feng-Yun
Department of Immunology , Haerbin Medical University , Haerbin 150086 , China
Abstract Objective The pathogenic mechanism of asthma has not yet been explained. This paper aims to study the relationships between IL-4, IL-10 and IFN-γ and asthma so as to explore the immunologic pathogenesis of asthma. Methods The Wistar rats were randomly assigned into 2 groups: a Normal group (n=11) and an Asthma group (n=11). Asthma was induced by intraperitoneal injection of OVA and fog inhalation of OVA. Five days after asthma induction, the rats were sacrificed and their spleens were removed. Then the mononuclear cell suspension was prepared, cultured, and stimulated with OVA. The levels of IL-4, IL-10 and IFN-γ in the supernatant were measured by enzyme linked immunosorbent assay (ELISA). Results The IL-4 level in the mononuclear cells of the Asthma group ( 64.56± 5.83 pg/ml) was significantly higher than that of the Normal group ( 24.66± 3.68 pg/ml) (P< 0.05), while the levels of IL-10 ( 34.13± 0.85 pg/ml) and IFN-γ ( 3.87± 0.48 pg/ml) were significantly lower than those of the Normal group [( 85.12± 6.13) and ( 14.51± 1.32) pg/ml, respectively] (P< 0.05). Conclusion IL-4, IL-10 and IFN-γ might be involved in the pathogenesis of asthma.
Key words ：
Asthma
Interleukin24
Interleukin210
Interferon2γ
Rat
Cite this article:
LI Guang,ZIAO Yu-Ying,QU Shu-Qiang et al. Levels of IL24 , IL210 and IFN2γ in rats with asthma[J]. 中国当代儿科杂志, 2004, 6(6): 474-476.
LI Guang,ZIAO Yu-Ying,QU Shu-Qiang et al. Levels of IL24 , IL210 and IFN2γ in rats with asthma[J]. CJCP, 2004, 6(6): 474-476.
URL:
http://www.zgddek.com/EN/ OR http://www.zgddek.com/EN/Y2004/V6/I6/474
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