OBJECTIVE: Nutrition insulin like growth factors (IGFs) axis is important to body catch up growth and gastrointestinal development, while gastrointestinal development is closely related to the nutritional absorb and catch up growth in infants with intrauterine growth retardation (IUGR). Now there are few reports about the postnatal intestinal development of IUGR infants in our country, and the reports have mainly focused on the gastrointestinal morphology and structure of new born IUGR rats. This research aims to study the effects of early postnatal diet with different levels of protein and caloric on serum IGF 1, IGFBP3, intestinal development and catch up growth in rats with IUGR. METHODS: The IUGR rat model was established by nutrition restriction. Sixty four IUGR pups were randomly assigned into four groups: the IUGR model group, the IUGR high protein diet group, the IUGR low protein diet group and the IUGR high caloric group. The rats in the four groups were fed with a normal protein, a 30% protein and a 10% protein diets, and a diet with caloric being higher by 20% than the other three groups respectively. Sixteen normal pups receiving normal diet were served as the control group. The serum concentrations of IGF 1 and IGFBP3, body weight, body length and intestinal weight and length were measured at the 4th and 12th weeks after birth respectively. RESULTS: The IUGR high protein diet and high caloric diet groups manifested quick intestinal development and catch up growth, as well as the increased IGFs level; the IGFs level of the IUGR high protein diet group at the 4th week after birth was significantly higher than the other groups (P< 0.05 ). The IUGR model group manifested a relatively slow intestinal development and catch up growth and a non increased IGFs level. The body weight, body length and intestinal weight and length in the IUGR low protein diet group at the 4th and 8th weeks after birth were lower than those of other groups and the IGFs level was lower at the 4th after birth compared with that of the other groups (P< 0.05 ). The IGFs level was positively correlated to body weight, body length and intestinal weight and length at the 4th after birth (r= 0.930 , 0.884 , 0.678 , 0.978 respectively, all P< 0.05 ). At the 12th week after birth, there was no correlation among them. CONCLUSIONS: IGF 1 is a sensitive index reflecting catch up growth and is positively related to intestinal development and body catch up growth during childhood (4th week of life), while the correlation disappears at adulthood (12th week of life)."/>
Relationship Between Insulin Like Growth Factors and Intestinal Development as Well as Physical Development in Rats with Intrauterine Growth Retardation
Relationship Between Insulin Like Growth Factors and Intestinal Development as Well as Physical Development in Rats with Intrauterine Growth Retardation
HUANG Ting-Ting, QIU Xiao-Shan, SHEN Zhen-Yu, KE Zhi-Yong, LAI Feng
Department of Pediatrics, First Affiliated Hospital of Zhongshan University, Guangzhou 510080, China
Abstract OBJECTIVE: Nutrition insulin like growth factors (IGFs) axis is important to body catch up growth and gastrointestinal development, while gastrointestinal development is closely related to the nutritional absorb and catch up growth in infants with intrauterine growth retardation (IUGR). Now there are few reports about the postnatal intestinal development of IUGR infants in our country, and the reports have mainly focused on the gastrointestinal morphology and structure of new born IUGR rats. This research aims to study the effects of early postnatal diet with different levels of protein and caloric on serum IGF 1, IGFBP3, intestinal development and catch up growth in rats with IUGR. METHODS: The IUGR rat model was established by nutrition restriction. Sixty four IUGR pups were randomly assigned into four groups: the IUGR model group, the IUGR high protein diet group, the IUGR low protein diet group and the IUGR high caloric group. The rats in the four groups were fed with a normal protein, a 30% protein and a 10% protein diets, and a diet with caloric being higher by 20% than the other three groups respectively. Sixteen normal pups receiving normal diet were served as the control group. The serum concentrations of IGF 1 and IGFBP3, body weight, body length and intestinal weight and length were measured at the 4th and 12th weeks after birth respectively. RESULTS: The IUGR high protein diet and high caloric diet groups manifested quick intestinal development and catch up growth, as well as the increased IGFs level; the IGFs level of the IUGR high protein diet group at the 4th week after birth was significantly higher than the other groups (P< 0.05 ). The IUGR model group manifested a relatively slow intestinal development and catch up growth and a non increased IGFs level. The body weight, body length and intestinal weight and length in the IUGR low protein diet group at the 4th and 8th weeks after birth were lower than those of other groups and the IGFs level was lower at the 4th after birth compared with that of the other groups (P< 0.05 ). The IGFs level was positively correlated to body weight, body length and intestinal weight and length at the 4th after birth (r= 0.930 , 0.884 , 0.678 , 0.978 respectively, all P< 0.05 ). At the 12th week after birth, there was no correlation among them. CONCLUSIONS: IGF 1 is a sensitive index reflecting catch up growth and is positively related to intestinal development and body catch up growth during childhood (4th week of life), while the correlation disappears at adulthood (12th week of life).
HUANG Ting-Ting,QIU Xiao-Shan,SHEN Zhen-Yu et al. Relationship Between Insulin Like Growth Factors and Intestinal Development as Well as Physical Development in Rats with Intrauterine Growth Retardation[J]. 中国当代儿科杂志, 2003, 5(6): 515-518.
HUANG Ting-Ting,QIU Xiao-Shan,SHEN Zhen-Yu et al. Relationship Between Insulin Like Growth Factors and Intestinal Development as Well as Physical Development in Rats with Intrauterine Growth Retardation[J]. CJCP, 2003, 5(6): 515-518.
