Abstract OBJECTIVE: To study the effect of L-arginine (L-Arg) on Pax2 expression in the kidneys of pup rats with intrauterine growth retardation (IUGR). METHODS: Pregnant rats were randomly assigned into three groups：normal, IUGR and L-Arg treated IUGR. The rats in the normal group were fed with ordinary forage (21% protein) during pregnancy. Those in the other two groups were fed with low diet forage (10% protein) during pregnancy. The L-Arg treated group was given drinking water containing L-Arg (200 mg/kg) daily during 21 days of lactation. Pax2 expression in renal tissues was measured with immunohistochemical staining and Western blot in pup rats of 7 days, 21 days, 2 months and 3 months old. RESULTS: The immunohistochemical staining showed that Pax2 was not expressed in the pup rats from the normal group at any time point. Pax2 positive cells were found in renal glomerulus and kidney tubules of 2-months- and 3-months-old rats from the IUGR and L-Arg treated groups. And Pax2 expression in 3-months-old rats was significantly higher than that in 2-months-old rats (P<0.05). L-Arg treatment decreased significantly the Pax2 expression in 2-months- and 3-months-old rats when compared with the untreated IUGR group (P<0.05). Western blot showed that Pax2 protein was not expressed in 7-days- and 21-days-old pup rats from three groups. Pax2 protein expression in 2-months- and 3-months-old pup rats from the IUGR and L-Arg treated groups increased significantly compared with normal controls. Pax2 protein expression in the pup rats from the L-Arg treated group was significantly lower than that in the untreated IUGR pup rats (P<0.01). CONCLUSIONS: Pax2 is expressed in the kidneys of IUGR rats during adulthood. L-Arg treatment can decrease the expression of Pax2.
GE Jian-Fang,XIE Zong-De,LI Wen et al. Effect of L-arginine on Pax2 expression in the kidneys of pup rats with intrauterine growth retardation[J]. 中国当代儿科杂志, 2011, 13(2): 145-148.
GE Jian-Fang,XIE Zong-De,LI Wen et al. Effect of L-arginine on Pax2 expression in the kidneys of pup rats with intrauterine growth retardation[J]. CJCP, 2011, 13(2): 145-148.
［1］Quaggin SE. Transcriptional regulation of podocyte specification and differentiation［J］.Microsc Res Tech, 2002, 57(4): 208-211.
［2］Dressler GR.Pax-2, kidney development, and oncogenesis［J］. Med Pediatr Oncol,1996, 27(5): 440-444.
3.0.CO;2-M target="_blank">
［3］Yang Y,Gubler MC, Beaufils H. Dysregulation of podocyte phenotype in idiopathic collapsing glomerulopathy and HIV-associated ephropathy［J］.Nephron, 2002, 91(3): 416-423.
［8］Bard JB. Growth and death in the developing mammalian kidney: signals，receptors and conversations［J］.Bioessays, 2002, 24(1): 72-82.
［9］James RG, Kamei CN, Wang Q, Jiang R, Schultheiss TM. Odd skipped related 1 is required for development of the metanephric kidney and regulates formation and differentiation of kidney precursor cells［J］. Development, 2006, l33(15): 2995-3004.
［10］Stayner CK, Cunliffe HE, Ward TA, Eccles MR. Cloning and characterization of the human PAX2 promoter［J］. J Biol Chem, 1998, 273(39): 25472-25479.
［16］Gil FZ, Lucas SR, Gomes GN, Cavanal Mde F, Goimbra TM. Effects of intrauterine food restriction and long-term dietary supplementation with L-arginine on age-related changes in renal function and structure of rats［J］. Pediatr Res, 2005, 57(5 Pt 1): 724-731.
