Abstract OBJECTIVE: To study the distribution characteristics of adiponectin gene +45 single nucleotide polymorphisms (SNP) in Chinese children, and to determine the role of adiponectin gene +45 polymorphisms in the pathogenesis of childhood obesity. METHODS: A total of 147 Chinese obese and 118 healthy children were randomly selected and enrolled to identify adiponectin gene SNP+45 polymorphism by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Plasma adiponectin levels were determined using ELISA. Waist circumference (WC), waist to hip ratio (WHR), percentage of body fat (%BF), systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting plasma glucose (FPG), serum triglycerides (TG), total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), plasma fasting insulin (FINS), and homeostasis model assessment of insulin resistance (HOMA-IR) were measured. RESULTS: The allelic frequency of adiponectin gene SNP+45 in children with obesity and healthy controls were 40.5% and 25.4%, respectively. There were significant differences in the distribution of genotypes and the allelic frequency between the two groups (P<0.05). The plasma adiponectin levels were significantly higher, in contrast, %BF, HOMA-IR, TC and LDL-C levels were significantly lower in obese children with TT genotype than those in obese children with TG or GG genotype. CONCLUSIONS: The adiponectin gene SNP+45 polymorphism may be associated with pathogenesis of obesity in children. T→G variance may be associated an increased risk of childhood obesity and result in a decreased level of adiponectin.
［3］Vionnet N, Hani EH, Dupont S,Gallina S, Francke S, Dotte S, et al. Genomewide search for type 2 diabetes-susceptibility genes in French whites: evidence for a novel susceptibility locus for early-onset diabetes on chromosome 3q27-qter and independent replication of a type 2-diabetes locus on chromosome 1q21-q24［J］. Am J Hum Genet, 2000, 67(6):1470-1480.
［4］Francke S, Manraj M, Lacquemant C, Lecoeur C, Leprêtre F, Passa P, et al. A genome-wide scan for coronary heart disease suggests in Indo-Mauritians a susceptibility locus on chromosome 16p13 and replicates linkage with the metabolic syndrome on 3q27［J］. Hum Mol Genet, 2001, 10（24）: 2751-2765.
［5］Chung HK, Chae JS, Hyun YJ, Paik JK, Kim JY, Jang Y, et al. Influence of adiponectin gene polymorphisms on adiponectin level and insulin resistance index in response to dietary intervention in overweight-obese patients with impaired fasting glucose or newly diagnosed type 2 diabetes［J］. Diabetes Care, 2009, 32(4): 552-558.
［8］Petrone A, Zavarella S, Caiazzo A, Leto G, Spoletini M, Potenziani S, et al. The promoter region of the adiponectin gene is a determinant in modulating insulin sensitivity in childhood obesity［J］. Obesity (Silver Spring), 2006, 14(9): 1498-1504.
［9］Bouatia-Naji N, Meyre D, Lobbens S. Séron K, Fumeron F, Balkau B, et al. ACDC/adiponectin polymorphisms are associated with severe childhood and adult obesity［J］. Diabetes, 2006, 55(2): 545-550.
