Abstract Objective To determine the frequencies and significance of myeloid-derived suppressor cells (MDSCs) and T-helper 17 (Th17) cells in peripheral blood of young children with recurrent wheezing. Methods Thirty young children with an acute exacerbation of recurrent wheezing were randomly enrolled. Twenty age-matched children with bronchopneumonia (pneumonia group) and 23 age-matched preoperative children with non-infectious or nonneoplastic diseases (hernia or renal calculus) (control group) were selected. The frequencies of MDSCs and Th17 cells in the peripheral blood were measured using flow cytometry and their correlation was determined by the Spearman’s correlation coefficient. Results The percentage of MDSCs in nucleated cells was significantly higher in the wheezing group than in the pneumonia and control groups (P<0.05), and it was significantly higher in the pneumonia group than in the control group (P<0.05). The percentage of Th17 cells in mononuclear cells was significantly higher in the wheezing group than in the pneumonia and control groups (P<0.05), but it showed no significant difference between the pneumonia and control groups (P>0.05). The frequency of MDSCs was positively correlated with the frequency of Th17 cells in the wheezing group (r=0.645, P<0.01). Conclusions MDSCs and Th17 cells may contribute to the pathogenesis of recurrent wheezing in young children.
About author:: 10.7499/j.issn.1008-8830.2015.04.004
Cite this article:
WANG Pan,LV Jian-Ping,LV Jin-Quan. Measurement of myeloid-derived suppressor cells and T-helper 17 cells in peripheral blood of young children with recurrent wheezing[J]. CJCP, 2015, 17(4): 312-316.
WANG Pan,LV Jian-Ping,LV Jin-Quan. Measurement of myeloid-derived suppressor cells and T-helper 17 cells in peripheral blood of young children with recurrent wheezing[J]. CJCP, 2015, 17(4): 312-316.
Stein RT, Martine FD. Asthma phenotypes in childhood: lessons from an epidemiological approach[J]. Peadiatr Respir Rev, 2004, 5(2): 155-161.
[2]
Bisgaard H, Szefler S. Prevalence of asthma-like symptoms in yong children[J]. Pediatr Pulmonol, 2007, 42(8): 723-728.
[3]
Young MR, Newby M, Wepsic HT, et al. Hematopoiesis and suppressor bone marrow cells in mice bearing large metastatic lewis lung carcinama tumors[J]. Cancer Res, 1987, 47(1): 100- 105.
[4]
Strobers. Natural suppressor (NS) cells, neonatal tolerance ,and total lympoid irradiation:exploring obscure relationship[J]. Annu Rev Immol, 1984, 4(2): 219-237.
[5]
Kao J, Ko EC, Eisenstein S, et al. Targeting immune suppressing myeloid-derived suppressor cells in oncology[J]. Crit Rev Oncol Hematol, 2011, 77(1): 12-29.
Cuenca AG, Delano MJ, Keuy-Scumpia KM, et al. A paradoxical role for myeloid-derived suppressor cell in sepsis and trauma[J]. Mol Med, 2011, 17(3-4): 281-292.
Kawaguchi M, Kokubu F, Fujita J, et al. Role of interleukin- 17in asthma[J]. Inflanm Allergy Drug Targets, 2009, 8(5): 383- 389.
[19]
Rodriguez PC, Hernandez CP, Quiceno D, et al. Arginase I in myeliod suppressor cells is induced by COX-2 in lung carcinoma[J]. Exp Med, 2005, 202(7): 931-939.
[20]
Veldhoen M, Hocking RT, Atkins CJ, et al. TGF-β in the context of an inflammatory cytokine milieu suppors denovo differentiation of IL-17-producing T cells[J]. Immunity, 2006, 24(2): 179-189.
[21]
Hoechst B, Gamrekelashvili J, Manns MP, et al. Plasticity of human Th17 cells and iTregs is orchestrated by different subsets of myeloid cells[J]. Blood, 2011, 117(24): 6532-6541.
[22]
Chtterjee S, Das S, Chakraborty P, et al. Myeloid derived suppressor cells (MDSCs) can induce the generation of Th17 response from naive CD4+T cells[J]. Immunobiology, 2013, 218(5): 718-724.
[23]
Yi H, Guo C, Yu X, et al. Mouse CD11b+Gr-1+++ myeloid cells can promote Th17 cell differentiation and experimental autoimmune encephalomyelitis[J]. J Immunol, 2012, 189(9): 4295-4304.
