Abstract Objective To study the role of serum neutrophil elastase (NE) level in acute exacerbation of asthma in preschool children. Methods A total of 85 preschool children who were diagnosed with asthma between January 2008 and January 2010 were classified into acute exacerbation group (n=44) and non-acute exacerbation group (n=41). Thirty-five children who received physical examination served as the control group. The enzyme-linked immunosorbent assay was used to determine the serum levels of NE and interleukin-8 (IL-8). The receiver operating characteristic (ROC) curve was used for NE evaluation. Results Both the acute and non-acute exacerbation groups had higher serum levels of NE and IL-8 than the control group, and the acute exacerbation group had significantly higher serum levels of NE and IL-8 than the non-acute exacerbation group (Pr=0.48, P27.73 μg/L as the cut-off value for diagnosing acute exacerbation of asthma, the sensitivity was 65.9%, the specificity was 95.1%, and the area under the ROC curve was 0.87 (PConclusions The determination of serum NE level in preschool children with asthma helps to diagnose the acute exacerbation of asthma.
YU Zhi-Wei,HUANG Song-Ming. Role of serum neutrophil elastase determination in the diagnosis of acute exacerbation of asthma in preschool children[J]. CJCP, 2016, 18(5): 396-399.
YU Zhi-Wei,HUANG Song-Ming. Role of serum neutrophil elastase determination in the diagnosis of acute exacerbation of asthma in preschool children[J]. CJCP, 2016, 18(5): 396-399.
Nair P, Aziz-Ur-Rehman A, Radford K. Therapeutic implications of 'neutrophilic asthma'[J]. Curr Opin Pulm Med, 2015, 21(1):33-38.
[2]
Mann BS, Chung KF. Blood neutrophil activation markers in severe asthma: lack of inhibition by prednisolone therapy[J]. Respir Res, 2006, 7(1): 59.
[3]
Baines KJ, Simpson JL, Wood LG, et al. Systemic upregulation of neutrophil α-defensins and serine proteases in neutrophilic asthma[J]. Thorax, 2011, 66(11): 942-947.
[4]
Ducharme FM, Dell SD, Radhakrishnan D, et al. Diagnosis and management of asthma in preschoolers: A Canadian Thoracic Society and Canadian Paediatric Society position paper[J]. Can Respir J, 2015, 22(3): 135-143.
[5]
Wood LG, Baines KJ, Fu J, et al. The neutrophilic inflammatory phenotype is associated with systemic inflammation in asthma[J]. Chest, 2012, 142(1): 86-93.
[6]
Chu YT, Chiang W, Wang TN, et al. Changes in serum eotaxin and eosinophil cationic protein levels, and eosinophil count during treatment of childhood asthma[J]. J Microbiol Immunol Infect, 2007, 40(2): 162-167.
Yoshihara S, Yamada Y, Abe T, et al. Association of epithelial damage and signs of neutrophil mobilization in the airways during acute exacerbations of paediatric asthma[J]. Clin Exp Immunol, 2006, 144(2): 212-216.
[9]
Lee TH, Song HJ, Park CS. Role of inflammasome activation in development and exacerbation of asthma[J]. Asia Pac Allergy, 2014, 4(4): 187-196.
[10]
Hosoki K, Ying S, Corrigan C, et al. Analysis of a Panel of 48 Cytokines in BAL Fluids Specifically Identifies IL-8 Levels as the Only Cytokine that Distinguishes Controlled Asthma from Uncontrolled Asthma, and Correlates Inversely with FEV1[J]. PLoS One, 2015, 10(5): e0126035.
[11]
Matsuoka H, Niimi A, Matsumoto H, et al. Inflammatory subtypes in cough-variant asthma: association with maintenance doses of inhaled corticosteroids[J]. Chest, 2010, 138(6): 1418-1425.
[12]
Simpson JL, Powell H, Boyle MJ, et al. Clarithromycin targets neutrophilic airway inflammation in refractory asthma[J]. Am J Respir Crit Care Med, 2008, 177(2): 148-155.
[13]
Brusselle GG, Vanderstichele C, Jordens P, et al. Azithromycin for prevention of exacerbations in severe asthma (AZISAST): a multicentre randomised double-blind placebo-controlled trial[J]. Thorax, 2013, 68(4): 322-329.
[14]
Tsai YF, Hwang TL. Neutrophil elastase inhibitors: a patent review and potential applications for inflammatory lung diseases (2010-2014)[J]. Expert Opin Ther Pat, 2015, 25(10): 1145-1158.
