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2010 Vol.  12 No.  02
Published: 2010-02-15
CLINICAL RESEARCH
EXPERIMENTAL RESEARCH
CLINICAL EXPERIENCE
CASE REPORT
REVIEW
CLINICAL RESEARCH
81 CAI Xiao-Di, CAO Yun, CHEN Chao, YANG Yi, WANG Chuan-Qing, ZHANG Lan, DING Hong
Investigation of nosocomial infection in the neonatal intensive care unit
OBJECTIVE: A perspective study was conducted to describe the epidemiologic profile of nosocomial infection in the neonatal intensive care unit (NICU). METHODS: The newborn infants who were admitted in the NICU for more than 48 hrs were enrolled from February 2006 to January 2007. The clinical data were collected. The rate of nosocomial infection was calculated according to the CDC surveillance system. The risk factors of nosocomial infection were investigated by multivariate regression analysis. RESULTS: A total of 1 159 neonates were recruited. A total of 169 nosocomial infections occurred, with a cumulative rate for nosocomial infection of 14.58%. The incidence of nosocomial infection was 19.52 per 1 000 patient-days. Ninety-two cases of pneumonia, including 38 cases of ventilator-associated pneumonia (VAP), were reported, with a nosocomial infection rate of 7.94%, which was the most common nosocomial infection in the NICU. Among these infants the rate of VAP was 48.8 per 1 000 ventilator days. The major microorganisms isolated from the infected patients were Acinetobacter baumannii, Klebsiella pneumoniae, Coagulase negative staphylococcus, and aeruginosus Bacillus. Birth weight (OR 2.130, 95%CI 1.466-3.094), mechanical ventilation (OR 7.038, 95%CI 3.901-12.698), chest tube drainage (OR 7.004, 95%CI 1.841-26.653) and ibuprofen therapy (OR 2.907, 95% CI 1.303-6.487) were the risk factors for the development of nosocomial infection. CONCLUSIONS: Pulmonary infection is the most common nosocomial infection in the NICU, and the Gram-negative bacillus is the main pathogen. Low birth weight, mechanical ventilation, chest tube drainage and ibuprofen therapy are independent risk factors for nosocomial infection in the NICU.［Chin J Contemp Pediatr, 2010, 12 (2):81-84］
2010 Vol. 12 (02): 81-84 [Abstract] ( 7412 ) [HTML 1KB] [PDF 1060KB] ( 2181 )
85 HUANG Yu-Jun, WU Shao-Guo, QU Xiao-Bing, ZHANG Li
Changes of iron metabolism indices in children with various genotypes of thalassema
OBJECTIVE: To study the value of iron metabolism indices, serum iron (SI), total iron blinding capacity (TIBC) and transferring (Tf), in thalassema. METHODS: The serum samples from 9 children with silent α thalassema, 56 with standard α thalassema, 26 with HbH disease, 40 with β+ thalassema, 56 with β0 thalassema, 45 with iron deficiency anemia (IDA) and 70 healthy children were detected for SI, TIBC and Tf levels. RESULTS: The SI level increased (P<0.01), while the TIBC level decreased significantly in the β0 thalassema group compared with those in the other groups (P<0.05 or 0.01), but the Tf level was not different. The Tf level of both the silent α thalassema and the standard α thalassema groups was statistically lower than that of the healthy group (P<0.01),but the levels of SI and TIBC were similar to the healthy group. Though the SI level of the HbH disease group was similar to the healthy group, the TIBC and Tf levels were statistically lower (P<0.01). CONCLUSIONS: Compared with Tf, SI and TIBC are better indices for monitoring iron loading in children with thalassema. The increased SI level and decreased TIBC level are two indices for the diagnosis of β0 thalassema in children with cellule anaemia.［Chin J Contemp Pediatr, 2010, 12 (2):85-88］
2010 Vol. 12 (02): 85-88 [Abstract] ( 6029 ) [HTML 1KB] [PDF 984KB] ( 1818 )
89 ZHOU Xi-Hui, MA Ai-Qun, LIU Xiao-Hong, HUANG Chen, ZHANG Yan-Min, SHI Rui-Ming
Linkage analysis and gene mapping of one Chinese family with benign familial infantile convulsions
OBJECTIVE: The present study performed linkage analysis and gene mapping to find the possible chromosome locus harboring in one family with benign familial infantile convulsions (BFIC) and investigate the possible molecular pathogenesis of BFIC. METHODS: A four-generation family with BFIC was investigated. The family was genotyped using eight hypervariable microsatellite markers covering four loci: D19S245 and D19S250 for the 19q12-13.1 region, D16S3131 and D16S3133 for the 16p12-q12 region, D2S156 and D2S286 for the 2q24 region, and D20S480 and D20S481 for the 20q13.3 region. Polymorphism fragments were amplified using polymerase chain reaction (PCR) method. PCR products for the markers were subjected to electrophoresis on 8% denatured polyacrylamide gel and silver staining for length judgment of amplification fragment. Linkage analysis was performed by use of MLINK in the LINKAGE computer package. Two-point LOD scores were calculated to estimate the linkage relationship. RESULTS: The two-point LOD scores were less than -2.0 for the genetic markers at chromosomes 19q12-13.1, 16p12-q12 and 2q24 at the recombination rate between 0.000 and 0.01. The two-point LOD scores for D20S481 at the 20q13.3 region were 0.3 and 0.25 at the recombination rate of 0.000 and 0.01, respectively. CONCLUSIONS: There is no evidence that this family with BFIC is linked to one of the following loci: 19q12-13.1, 16p12-q12 and 2q24, but a possible linkage with 20q13.3 region cannot be excluded.［Chin J Contemp Pediatr, 2010, 12 (2):89-92］
2010 Vol. 12 (02): 89-92 [Abstract] ( 5549 ) [HTML 1KB] [PDF 1057KB] ( 1447 )
93 WANG Lian, DENG Yan-Chun, LIU Yong-Hong, HUANG Yuan-Gui
Characteristics of continuous spike-and-wave during slow wave sleep syndrome in children
OBJECTIVE: Continuous spike-and-wave during slow wave sleep (CSWS) syndrome is one of the presentations of electrical status epilepticus during sleep (ESES). The purpose of this study was to investigate the characteristics of CSWS syndrome in children. METHODS: Between 2007 and 2009, a total of 778 nocturnal long-term or 24-hr video-EEG records were included. The EEG, clinical and neuroimaging characteristics were studied in children who met standard criteria for CSWS. RESULTS: Nine children met standard criteria for CSWS in video-EEGs. Their ages ranged 6 to 13 years. Their EEGs were characterized by continuous spike-and-wave (SW) discharges during non-rapid eye movement (NREM) sleep, accounting for 85%-100% of the period of NREM sleep. Clinically, these children had various types of epileptic seizures and exhibited different degrees of neuropsychiatric impairments, language dysfunction, and/or behavioral disturbances. Neuroimaging abnormalities were found in 6 cases, including atelencephalia or atrophy, gray matter heterotopia and leucomalacia. CONCLUSIONS: This study indicates the characteristics of CSWS syndrome in clinical manifestations, EEG and neuroimaging examinations. This will be helpful in understanding CSWS syndrome.［Chin J Contemp Pediatr, 2010, 12 (2):93-95］
2010 Vol. 12 (02): 93-95 [Abstract] ( 6082 ) [HTML 1KB] [PDF 1070KB] ( 1846 )
96 ZHANG Jing, WANG Kai-Xuan, WEI Yi, XU Min-Hui, SU Jin-Mei, BAO Yun-Guang, ZHAO Shi-Yong
Effect of topiramate and carbamazepine on bone metabolism in children with epilepsy
OBJECTIVE: To assess bone health in epileptic children who have been treated with topiramate (TPM) or carbamazepine (CBZ). METHODS: Sixty-three epileptic children who received TPM or CBZ treatment and 36 eileptic children who did not receive any drug treatment (control group) were enrolled. Bone mineral density (BMD) at lumbar vertebrae (L1-L4) and radius-ulna was evaluated by the dual-energy X-ray absorptiometry method. Biochemical indices of bone metabolism, including serum calcium, phosphorus and alkaline phosphatase contents were measured. RESULTS: The serum calcium content was higher in the TPM group (2.41±0.17 mmol/L), but it was lower in the CBZ group (2.15±0.26 mmol/L) than that (2.26±0.11 mmol/L) in the control group (P<0.05). The serum phosphorus content in both the TPM (1.55±0.17 mmol/L) and the CBZ groups (1.52±0.26 mmol/L) was significantly lower than that in the control group (1.70±0.30 mmol/L) (P<0.05). There were no significant differences in the serum content of alkaline phosphatase between three groups. BMD was significantly reduced in both the TPM and the CBZ groups when compared to the control group (P<0.05). CONCLUSIONS: TPM and CBZ may result in alterations in serum contents of calcium, phosphorus and alkaline phosphatase as well as BMD reduction.［Chin J Contemp Pediatr, 2010, 12 (2):96-98］
2010 Vol. 12 (02): 96-98 [Abstract] ( 5549 ) [HTML 1KB] [PDF 974KB] ( 1478 )
99 YANG Sen, ZHU Qi, CHEN Jiao, HUA Yi-Min, ZHOU Kai-Yu, GUO Nan
Echocardiography diagnosis of fetal cardiac structural abnormalities
OBJECTIVE: To investigate the application of echocardiography diagnosis of fetal cardiac structural abnormalities. METHODS: The echocardiography findings of 9 352 fetus were studied. RESULTS: A total of 472 cases showed cardiac structural abnormalities, including 7 cases of ventricular septal defect, 53 cases of atrioventricular septal defect, 49 cases of atrial septal defect, 26 cases of tetralogy of Fallot, 21 cases of persistent truncus arteriosus, 20 cases of Ebstein's anomaly and 206 cases of other cardiac abnormalities. There were 17 cases with cardiac arrhythmia, 9 with heart failure, and 5 with hydrops. CONCLUSIONS: Fetal echocardiography is a promising diagnostic tool for prenatal evaluation of cardiac structural abnormalities. The echocardiography diagnosis and evaluation for fetal congenital cardiovascular malformations is the foundation of the guidance and monitoring in intrauterine fetal cardiac intervention.［Chin J Contemp Pediatr, 2010, 12 (2):99-102］
2010 Vol. 12 (02): 99-102 [Abstract] ( 6253 ) [HTML 1KB] [PDF 980KB] ( 2584 )
103 MENG Xiang-Chun, CAI Hua-Bo, LI Zhi-Chuan, WANG Tao, ZHANG Qing, ZHANG Zhi-Wei
A modified management of the transcatheter occlusion of patent ductus arteriosus: using angiography combined with transthoracic echocardiography
OBJECTIVE: To evaluate the feasibility of angiography combined with transthoracic echocardiography (TEE) as a modified management of the transcatheter occlusion of patent ductus arteriosus (PDA). METHODS: Forty children with PDA were randomly divided into two groups (n=20 each): observed and control. The control group accepted traditional transcatheter occlusion, and the observed group received a modified management (angiography combined with TEE). The children in the observed group were monitored by realtime TTE. RESULTS: A complete occlusion was acquired by one occlusion operation in each child in the observed group. The TTE demonstrated that the occlusion device was in place, and that the blood flow velocities in the left and right pulmonary artery and the descending aorta were in normal ranges. There were shorter X-ray exposure time, shorter recovering time and less ICU stay time in the observed group than in the control group. The complications associated with blood vessel puncturation occurred in four children from the control group, but none of the observed group had the complications. The total hospitalization cost in the observed group was less than in the control group. CONCLUSIONS: Angiography combined with TEE as a modified management of the transcatheter occlusion of PDA is recommended.［Chin J Contemp Pediatr, 2010, 12 (2):103-105］
2010 Vol. 12 (02): 103-105 [Abstract] ( 5552 ) [HTML 1KB] [PDF 963KB] ( 1498 )
106 MA Jing, HUANG Chun-Xiang, PI Pi-Xiang, XIA Ai-Bin, SU Lin-Yan
DRD2/ANKK1 Taq IA polymorphism and early infant temperament
OBJECTIVE: To investigate whether there is an association between DRD2/ANKK1 Taq IA polymorphism and early infant temperament. METHODS: DRD2/ANKK1 Taq IA polymorphism (rs1800497) was determined using polymerase chain reaction-ligase detection reaction (PCR-LDR) techniques in 149 Chinese Han infants from Changsha City. Their mothers were asked to complete the Early Infant Temperament Questionnaires (EITQ) when the infants were 1 to 4 months old (mean: 2.75 months). There were three genotypes found in these infants: C/C, T/T and C/T. The subjects were subdivided into T-carrier (CT, TT) and non-T-carrier (CC) groups for statistical analysis. RESULTS: There were no differences in the temperament style distribution between the T-carrier and non-T carrier groups. There were also no statistically significant differences between the two groups in the score of the nine temperament dimensions. CONCLUSIONS: DRD2/ANKK1 Taq IA polymorphism is not associated with early infant temperament.［Chin J Contemp Pediatr, 2010, 12 (2):106-109］
2010 Vol. 12 (02): 106-109 [Abstract] ( 6015 ) [HTML 1KB] [PDF 995KB] ( 1336 )
110 LIU Wei-Ping, SUN Su-Fang, ZHANG Gui-Li
Expression of apoptosis-related proteins in gastric mucosa of children with Helicobacter pylori infection
OBJECTIVE: To investigate the relationship between apoptosis-related proteins in gastric mucosa, p53 and Bax, and Helicobacter pylori (H. pylori) infection in children. METHODS: p53 and Bax expression in gastric mucosa were measured using immunohistochemical technique in 33 children with gastric mucosal lesions. Presence/absence of H. pylori infection was detected by the rapid urease and pathological tests. RESULTS: Fifteen children (88%) showed positive expression of p53 in 17 children who were confirmed with H. pylori infection, compared with 9 (56%) in 16 H. pylori negative children. Thirteen children (76%) showed positive expression of Bax in the 17 children with H. pylori infection, compared with 6 (38%) in the 16 H. pylori negative children. The expression levels of p53 and Bax in the H. pylori positive group were significantly higher than those in the H. pylori negative group (P<0.05). CONCLUSIONS: H. pylori infection is associated with the over-expression of p53 and Bax proteins in gastric mucosa in children.［Chin J Contemp Pediatr, 2010, 12 (2):110-112］
2010 Vol. 12 (02): 110-112 [Abstract] ( 5154 ) [HTML 1KB] [PDF 1131KB] ( 1314 )
113 WANG Xiu-Fang, YANG Jin-Ling, QIAO Jun-Ying, ZHANG Yan-Li
Measurement of eosinophils and interleukin-17 in nasopharyngeal secretions of children under 5 years old with wheezing
OBJECTIVE: To study the value of eosinophils (EOS) and interleukin-17 (IL-17) in nasopharyngeal secretions in the evaluation of progress of wheezing in children under 5 years old. METHODS: Fifty-three children under five years old who had recurrent wheezing were classified into two groups: wheezing group I with atopic body (n=27) and wheezing group II without atopic body (n=26). Twenty pre-surgical children with non-infectious disease were used as the control group. Nasopharyngeal secretions were collected. Inflammatory cells in nasopharyngeal secretions were counted under the microscope. IL-17 levels in supernatants were measured using ELISA. RESULTS: EOS counts in nasopharyngeal secretions in wheezing group I were significantly higher than those in wheezing group II and the control group (P<0.05, P<0.01, respectively). There were no significant differences in EOS counts between wheezing II and the control groups. The IL-17 levels in both wheezing groups were significantly higher than those in the control group (P<0.01), and the wheezing group I had increased IL-17 levels than wheezing group II (1 474±974 pg/mL vs 788±132 pg/mL; P<0.05). The IL-17 level was positively correlated with the EOS counts in wheezing group I (r=0.62, P<0.05). CONCLUSIONS: EOS counts and IL-17 levels in nasopharyngeal secretions may be used as indices for identifying the tendency to develop asthma in children under 5 years old with wheezing.［Chin J Contemp Pediatr, 2010, 12 (2):113-116］
2010 Vol. 12 (02): 113-116 [Abstract] ( 4836 ) [HTML 1KB] [PDF 985KB] ( 1481 )
117 MA Ming-Sheng, ZOU Li-Ping, DING Ying-Xue, JIN Hong
Concentrations of granulocyte colony-stimulating factor in children with moyamoya disease
OBJECTIVE: To explore the roles of granulocyte colony-stimulating factor in the pathogenesis of moyamoya disease. METHODS: Serum G-CSF concentrations were measured using enzyme linked immunosorbent assay (ELISA) in 20 children with moyamoya disease and 20 healthy children. RESULTS: Serum G-CSF concentration (35.7±10.3 pg/mL) in children with moyamoya disease was significantly higher than that in healthy controls (23.5±3.8 pg/mL) (P<0.01). CONCLUSIONS: The elevated serum G-CSF concentration in children with moyamoya disease suggests that G-CSF may play an important role in the pathogenesis of moyamoya disease.［Chin J Contemp Pediatr, 2010, 12 (2):117-119］
2010 Vol. 12 (02): 117-119 [Abstract] ( 4666 ) [HTML 1KB] [PDF 975KB] ( 1266 )
120 ZHENG Wen-Jie, CHEN Min-Guang, CHEN Xiao-Ying, YANG Qing, LIN Rui-Xia
Renal expression of macrophage migration inhibitory factor in children with Henoch-Sch-nlein purpura nephritis
OBJECTIVE: To examine the expression of macrophage migration inhibitory factor (MIF) in renal tissues obtained from children with Henoch-Sch-nlein purpura nephritis (HSPN). METHODS: The renal tissue samples were obtained from 11 children with different pathological grades of HSPN and 8 children with thin glomerular basement membrane disease (controls). The MIF expression was measured by immunohistochemistry. The correlation between MIF expression and 24 hrs urinary protein excretions was evaluated using a linear correlation analysis. RESULTS: MIF expression was seldom found in renal tissues obtained from controls. However, a significantly increased MIF expression was found and was concordant with the increased severity of renal pathology in renal tissues obtained from children with HSPN. The MIF expression in renal tissues of grade Ⅲ-Ⅳ of renal pathology was significantly higher than that in gradeⅠ-Ⅱ in children with HSPN (P<0.01). In children with HSPN, there was an increased MIF expression in renal tissues with crescent formation and inflammatory cell infiltration. Renal MIF expression was significantly positively correlated with 24 hrs urinary protein excretions in children with HSPN (P<0.01). CONCLUSIONS: MIF may play an important role in renal injury of HSPN. Up-regulation of MIF expression may reflect the degree of renal lesions in HSPN.［Chin J Contemp Pediatr, 2010, 12 (2):120-122］
2010 Vol. 12 (02): 120-122 [Abstract] ( 5054 ) [HTML 1KB] [PDF 1159KB] ( 1412 )
CASE REPORT
119 FENG Yong-Ge, LIU Shu-Gang, GUO Xian-Su, WANG Hong-Yan
Digestive malformations in a neonate presenting with intractable diarrhea
No abstvact available
2010 Vol. 12 (02): 119-119 [Abstract] ( 3526 ) [HTML 1KB] [PDF 935KB] ( 1253 )
154 ZHOU Wen-Li, *Zhu-Min-Li, *Yan-Chao-Ying, LIN Zhen-Lang, LIU Xue-Hua
Neonatal sepsis caused by listeria monocytogenes: case report of 7 cases

No abstract available
2010 Vol. 12 (02): 154-155 [Abstract] ( 3914 ) [HTML 1KB] [PDF 942KB] ( 1521 )
156 ZHANG He-Cheng, BAI Yan, WANG Rong-Feng, CHEN Zheng-Guang
Huge pneumatocele in a child

No abstract available
2010 Vol. 12 (02): 156-157 [Abstract] ( 4006 ) [HTML 1KB] [PDF 1007KB] ( 1341 )
157 XUE Zhi-Hua
3-methylc-rotonyl-CoA carboxylase deficiency: a case report

No abstract available
2010 Vol. 12 (02): 157-158 [Abstract] ( 4980 ) [HTML 1KB] [PDF 945KB] ( 1484 )
CHILD HEALTH CARE
123 GUAN Bing-Qing, LUO Xue-Rong, DENG Yun-Long, WEI Zhen, YE Hai-Sen, YUAN Xiu-Hong, NING Zhi-Jun, YANG Wei, DING Jun
Prevalence of psychiatric disorders in primary and middle school students in Hunan Province
OBJECTIVE: To investigate the prevalence of psychiatric disorders in a representative sample of primary and middle school students in Hunan Province. MethodsA total of 9 495 children aged 5-17 years from Hunan urban and rural schools were enrolled by a cluster sampling and a two-phase design. The students’ psychiatric status was assessed using the Investigation Screening Inventory for Child Mental Disorder and a semi-structured interview designed based on the DSM-IV criteria. RESULTS: The overall prevalence of psychiatric disorders was 16.22%. Attention-deficit and disruptive behavior disorders were the commonest in the diagnostic categories of psychiatric disorders (10.69%). Regarding specific disorders, the most prevalent was attention-deficit/hyperactivity disorder (5.95%). Psychiatric disorders were more prevalent in boys than in girls (20.49% vs 11.16%; P<0.01). The prevalence of attention-deficit and disruptive behavior disorders in boys was higher than in girls (14.76% vs 5.87%; P<0.01). The prevalence of psychiatric disorders in middle school students (12-17 years) was significantly higher than in primary students (5-11 years) (18.38% vs 14.64%; P<0.01). There were no significant differences in the prevalence of psychiatric disorders between urban and rural students. CONCLUSIONS: Psychiatric disorders are common among primary and middle school students in Hunan Province. The prevalence of this disorder in boys is higher than in girls. The middle school students have higher prevalence than primary students.［Chin J Contemp Pediatr, 2010, 12 (2):123-127］
2010 Vol. 12 (02): 123-127 [Abstract] ( 9779 ) [HTML 1KB] [PDF 1062KB] ( 1463 )
EVIDENCE-BASED MEDICINE
128 DAI Jing, HAN Lu, WANG Li, ZHANG Ling-Li
Levetiracetam therapy for childhood epilepsy: a systematic review
OBJECTIVE: Levetiracetam has been widely used for childhood epilepsy, but there is no high quality evidence to support its use. This study performed a systematic review to evaluate the effectiveness and safety of levetiracetam therapy for childhood epilepsy. METHODS: The papers related to levetiracetam therapy for childhood epilepsy published up to March, 2009 were retrieved electronically from the PubMed, Embase, the Cochrane Library, Chinese Biomedical Database, Wanfang and Weipu Chinese Journals Full-text Database. The relevant papers on randomized control trials (RCTs) or quasi-RCTs were studied by meta analysis. RESULTS: Two papers that met the inclusion criteria were included. The first paper involved 198 patients, including 108 cases in the levetiracetam therapy group and 97 cases in the placebo group. Seven cases (6.9%) were seizurefree in the levetiracetam therapy group compared with 1 case (1%) in the placebo group (P<0.01) 14 weeks after treatment. Levetiracetam therapy decreased significantly the frequency of seizures compared with the placebo treatment. The second paper involved 39 patients, including 21 cases in the levetiracetam therapy group and 18 cases in the oxcarbazepine therapy group. Nineteen cases (90.5%) were seizure-free in the levetiracetam therapy group compared with 13 cases (72.2%) in the oxcarbazepine therapy group (P=0.410) during a follow-up of 12-24 months. The adverse effects in the levetiracetam therapy group were not significantly different from the placebo and the oxcarbazepine therapy groups. CONCLUSIONS: The current evidence shows that levetiracetam therapy is effective for childhood epilepsy. However, it needs to be proved by the multi-centre, large sample RCTs.［Chin J Contemp Pediatr, 2010, 12 (2):128-131］
2010 Vol. 12 (02): 128-131 [Abstract] ( 6571 ) [HTML 1KB] [PDF 991KB] ( 1981 )
EXPERIMENTAL RESEARCH
132 JIA Sheng-Hua, WEI Hong, YU Jia-Lin, WEI Xiao-Di, ZHANG Xiao-Ping, LI Jin-Chun
Protective effects of curcumin on neonatal rats with necrotizing enterocolitis
OBJECTIVE: This study examined the effects of curcumin on intestinal histopathological changes, cyclooxygenase-2 (COX-2) expression, and tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) concentrations in neonatal rats with necrotizing enterocolitis (NEC) , in order to investigate the effects of curcumin against NEC. METHODS: Forty neonatal rats were randomly divided into four groups (n=10 each): normal control, solvent control, NEC model, and curcumin intervention. The general situations of rats were observed for 3 consecutive days, and the rats were then sacrificed on the 4th day. Intestinal tissues were obtained for examining the histopathological changes, COX-2 expression, and TNF-α and IL-10 concentrations. RESULTS: Curcumin treatment ameliorated the general situations and histopathological signs in rats with NEC. TNF-α and IL-10 concentrations in the NEC model and the curcumin intervention groups increased significantly compared with those in the normal and solvent control groups (P<0.05). The concentration of TNF-α decreased (P<0.05), while the concentration of IL-10 increased significantly in the curcumin intervention group in comparison with the NEC model group (P<0.05). Immunohistochemistry results indicated that the positive expression of COX-2 in the curcumin intervention group was significantly lower than that in the NEC model group. CONCLUSIONS: Curcumin has protective effects against NEC in neonatal rats, possibly through inhibiting COX-2 expression, reducing TNF-α content, and increasing IL-10 content.［Chin J Contemp Pediatr, 2010, 12 (2):132-136］
2010 Vol. 12 (02): 132-136 [Abstract] ( 5824 ) [HTML 1KB] [PDF 1318KB] ( 1541 )
137 LI Xiao-Hui, LUAN Bin
Effect of epidermal growth factor receptor on airway remodeling in asthmatic mice and its mechanism
OBJECTIVE: To explore the relationship of airway remodeling with epidermal growth factor receptor (EGFR) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) levels in asthmatic mice and the effect of EGFR tyrosine kinase inhibitor (AG1478) on airway remodeling. METHODS: Twenty-four male BALB/c mice were randomly divided into three groups: normal control, asthma, AG1478-treated. Mice were sensitized and challenged with ovalbumin (OVA) and a mouse mode1 of asthma was prepared. Collagen deposition was determined in Masson-stained lung sections. Periodic acid Schiff (PAS) staining was used to observe the proliferation of goblet cells. Immunohistochemistry was used to determine the protein expression of HB-EGF. RT-PCR was used to determine the mRNA expression of HB-EGF and EGFR. RESULTS: The characteristic changes of airway remodeling occurred in the asthma group. The expression of HB-EGF and EGFR in the epithelial cells of bronchi in the asthma group was significantly higher than that in the normal control group. Compared with the asthma group, the AG1478-treated group had decreased inflammation reactions, decreased collagen deposition and proliferation of goblet cells and lower expression of EGFR and HB-EGF. CONCLUSIONS: EGFR tyrosine kinase inhibitor (AG1478) ameliorates the progression of airway remodeling in mice with asthma by inhibitions of EGFR and HB-EGF expression and EGFR signal pathway.［Chin J Contemp Pediatr, 2010, 12 (2):137-140］
2010 Vol. 12 (02): 137-140 [Abstract] ( 5241 ) [HTML 1KB] [PDF 1453KB] ( 1491 )
CLINICAL EXPERIENCE
141 ZHANG Quan, WU Xiao-Lin
Prognosis of children with sepsis complicated by gastrointestinal dysfunction

No abstract available
2010 Vol. 12 (02): 141-142 [Abstract] ( 3398 ) [HTML 1KB] [PDF 954KB] ( 1235 )
143 ZHAO Sheng-Kui, WEI Xiao-Ming
Chinical analysis of 15 cases of abdominal compartment syndrome

No abstract available
2010 Vol. 12 (02): 143-144 [Abstract] ( 3807 ) [HTML 1KB] [PDF 941KB] ( 1219 )
145 ZHONG Tian-Ying, CHEN Qian, HU Zheng
Detection of enterovirus RNA by fluorescent quantitative PCR in children with hand-foot-mouth disease

No abstract available
2010 Vol. 12 (02): 145-146 [Abstract] ( 4302 ) [HTML 1KB] [PDF 960KB] ( 1928 )
147 CHEN Guo-Bing, WU Jin-Zhun, ZHUANG De-Yi, DING Xue-Qin
Diagnosis and treatment of severe hand-foot-mouth disease: experience of 33 cases

No abstract available
2010 Vol. 12 (02): 147-148 [Abstract] ( 4550 ) [HTML 1KB] [PDF 948KB] ( 1638 )
149 BAO Li-Ya, LI Yuan-Yuan, TIAN Miao, ZHANG Li-Sha, XIAO Jun, XIE Yuan
Investigation on hepatitis B virus infection in children from Guiyang area

No abstract available
2010 Vol. 12 (02): 149-150 [Abstract] ( 3771 ) [HTML 1KB] [PDF 945KB] ( 1083 )
151 XIE Lu, LUO Fen-Ping, ZHANG Xiu-Ya, ZHANG Li
Efficacy of low-dose oral erythromycin as an adjuvant therapy for cytomegalovirus infection

No abstract available
2010 Vol. 12 (02): 151-153 [Abstract] ( 3872 ) [HTML 1KB] [PDF 971KB] ( 1374 )
REVIEW
159 LIU Zhan-Li, ZOU Li-Ping
Research advance in the etiology of West syndrome

No abstract available
2010 Vol. 12 (02): 159-161 [Abstract] ( 4513 ) [HTML 1KB] [PDF 979KB] ( 1579 )
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