CJCP
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2012 Vol.  14 No.  07
Published: 2012-07-15
CLINICAL RESEARCH
EXPERIMENTAL RESEARCH
CLINICAL EXPERIENCE
CASE REPORT
CLINICAL RESEARCH
481 ZHANG Zhan-Zhan, CHENG Qian
A prospective study of late preterm infants' physical growth
OBJECTIVE: To evaluate birth weights (BW) in late preterm infants (LPI) with different gestational ages (GA) and their physical growths at correct ages (CA) 1, 3 and 6 months. METHODS: A total of 287 LPIs were recruited for a cohort study between May 2010 and September 2011. Birth weights (BW) in infants with different gestational ages and their physical growths at CA 1, 3 and 6 months were evaluated. RESULTS: Of the 287 LPIs, mean BW in LPIs with gestational ages of 34-34+6and 35-35+6weeks was significantly lower than the reference value from a physical growth investigation on newborns of 15 cities of China in 1986 (P<0.05). However, mean BW in singleton LPIs (n=128) with gestational ages of 36-36+6weeks was significantly higher than the reference value (P<0.05). At CA 1, 3 and 6 months, more than 90% LPIs reached P3 level in weights for age, length for age and head circumference for age. At CA 1 and 3 months, weight for age and head circumference for age in the LPIs were statistically higher than the reference values of physical growth in children under 7 years from nine cities of china in 2005 (P<0.05). Mean length for age at CA 1, 3 and 6 months was not significantly different from the reference value. CONCLUSIONS: There are obvious differences in BW in the LPIs compared with the reference value from a physical growth investigation on newborns of 15 cities of China in 1986, suggesting that the reference value of BW at different gestational ages needs to be updated in a certain interval. Before CA 6 months, there is a risk of overweight in LPIs and long-term monitoring is necessary. Growth of length should be followed-up for a long time.
2012 Vol. 14 (07): 481-484 [Abstract] ( 4882 ) [HTML 1KB] [PDF 1012KB] ( 1502 )
485 XIE Li-Juan, LI Hua-Jun, ZHU Jian-Xing
Relationship between serum S100B protein level and brain damage in preterm infants
OBJECTIVE: To study changes of serum S100B protein level in preterm infants with brain damage and its role. METHODS: Forty-seven preterm infants were classified into 3 groups based on the results of brain ultrasound and MRI: brain white matter damage (WMD; n=13), brain but not white matter damage (non-WMD; n=14) and control (no brain damage; n=20). Blood samples were collected within 24 hrs, 72 hrs and 7 days after birth. S100B protein level was measured using ELISA. RESULTS: Serum levels of S100B in the WMD and non-WMD groups were significantly higher than in the control group within 24 hours, 72 hours and 7 days after birth (P<0.05). More increased serum S100B levels were observed in the WMD group compared with the non-WMD group (P<0.05). CONCLUSIONS: Serum S100B protein level increases in preterm infants with brain damage within 7 days after birth, suggesting that it may be used as an early sensitive marker for the diagnosis of brain damage, especially WMD.
2012 Vol. 14 (07): 485-488 [Abstract] ( 4415 ) [HTML 1KB] [PDF 1302KB] ( 1200 )
489 ZHANG Jing, MAO Jian, LI Juan, CHEN Dan
MRI findings of neonatal purulent meningitis caused by different pathogenic bacteria
OBJECTIVE: To study the image characteristics of neonatal purulent meningitis caused by different pathogenic bacteria, using conventional magnetic resonance imaging (MRI). METHODS: The clinical data of 58 hospitalized newborns with purulent meningitis receiving MRI examination from January 2005 to February 2012 were retrospectively studied. Pathogens and MRI findings were investigated. RESULTS: Of the 58 newborns, 44 (76%) showed complications of the neural system on the first MRI. Escherichia coli were found as the most common pathogen in newborns with Gram-negative bacteria-induced purulent meningitis (six cases). Of the six cases, four presented with ventriculitis, one with subdural effusion and one with brain infarction on MRI. Listeria monocytogenes was frequently seen in newborns with Gram-positive bacteria-induced purulent meningitis (three cases). All three cases presented with different degrees of white matter damage on MRI. CONCLUSIONS: Neonatal purulent meningitis caused by different pathogenic bacteria has different image characteristics. This suggests that understanding the patterns of MRI findings is useful in the clinical diagnosis and treatment of neonatal purulent meningitis caused by different pathogens.
2012 Vol. 14 (07): 489-495 [Abstract] ( 6800 ) [HTML 1KB] [PDF 1727KB] ( 2134 )
496 LI Min, XIONG Ying, MU De-Zhi, WANG Hua
Changes to IFN-γ and IP-10 in the arterial blood of premature infants before and after mechanical ventilation
OBJECTIVE: This study examined changes to pro-inflammatory cytokine IFN-γ and chemokine IP-10 in the arterial blood of premature infants before and after mechanical ventilation, with the aim of exploring possible mechanisms of ventilation-induced lung injury. METHODS: Twenty-three neonates requiring mechanical ventilation were enrolled in this study. Arterial blood samples were collected for measuring IFN-γ and IP-10 levels using ELISA before and 4 hours after mechanical ventilation. RESULTS: Blood IFN-γ and IP-10 increased significantly from 59±40 pg/mL and 130±67 pg/mL respectively before mechanical ventilation to 105±54 pg/mL and 220±80 pg/mL respectively after 4 hours of mechanical ventilation (P<0.01). CONCLUSIONS: Serum IFN-γ and IP-10 levels increase after mechanical ventilation, suggesting that both may participate in the immune-inflammatory process of ventilation-induced lung injury.
2012 Vol. 14 (07): 496-498 [Abstract] ( 3970 ) [HTML 1KB] [PDF 927KB] ( 1046 )
499 CHEN Dan, HUANG Xi-Lin, LI Xiao-Ping
Clinical application of high-frequency oscillatory ventilation for the treatment of neonatal pneumothorax
OBJECTIVE: To evaluate the clinical effect of high-frequency oscillatory ventilation (HFOV) for the treatment of neonatal pneumothorax. METHODS: Retrospective analysis was performed on the clinical data of 23 neonates with pneumothorax who received HFOV from January 2007 to June 2011. Of the 23 cases, 19 cases were treated by HFOV as soon as they were diagnosed with pneumothorax, and 4 cases were treated by HFOV after the occurrence of pneumothorax during conventional mechanical ventilation (CMV) or continuous positive airway pressure (CPAP) ventilation. Another 23 neonates with pneumothorax who received CMV in the same period were selected as controls. The HFOV group and control group were compared with respect to oxygenation index (OI) and arterial/alveolar oxygen tension ratio (a/APO2) before and after 1, 12, 24, and 48 hours of ventilation as well as mechanical ventilation time, gas absorption time, complication, and prognosis. RESULTS: Both groups showed significantly decreased OI and significantly increased a/APO2 after ventilation (P<0.05). Compared with the control group, the HFOV group had significantly lower OI and significantly higher a/APO2 after 1, 12, 24, and 48 hours of ventilation (P<0.05). Mechanical ventilation and gas absorption times were significantly shorter in the HFOV group than in the control group (P<0.05). Twenty-two cases were cured in the HFOV group and 21 in the control group. Each group included one case of ventilator-associated pneumonia that was later cured with antibiotics. CONCLUSIONS: Compared with CMV, HFOV performs better in improving the pulmonary oxygenation function of neonates with pneumothorax and can shorten both mechanical ventilation time and gas absorption time without increasing the incidence of adverse effects.
2012 Vol. 14 (07): 499-501 [Abstract] ( 5306 ) [HTML 1KB] [PDF 923KB] ( 1661 )
502 LIN Xin-Zhu, CHEN Han-Qiang, ZHENG Zhi, LI Ya-Dan, LAI Ji-Dong, HUANG Li-Han
Therapeutic effect of early administration of oral ibuprofen in very low birth weight infants with patent ductus arteriosus
OBJECTIVE: To study therapeutic effect and safety of early administration of oral ibuprofen in very low birth weight infants (VLBWIs) with patent ductus arteriosus (PDA). METHODS: A total of 64 symptomatic VLBWIs (within 24 hours after birth) with PDA confirmed by bedside Color Doppler ultrasound were randomly divided into two groups: treatment and control (n = 32 each). The treatment group was orally administered ibuprofen within 24 hours after birth at 10 mg/kg, followed 24 hours later by a second dose of 5 mg/kg and 48 hours later by a third dose of 5 mg/kg. The control group was treated with placebo (normal saline) at 1 mL/kg, followed 24 hours later by a second dose of 0.5 mL/kg and 48 hours later by a third dose of 0.5 mL/kg. The therapeutic efficacies and adverse effects in both groups were observed. RESULTS: The treatment group showed a significantly higher closure rate of ductus arterious than the control group after one course of treatment (84% vs 41%; P0.05). No obvious adverse effects were observed in both groups. CONCLUSIONS: Early administration of oral ibuprofen for treatment of PDA in VLBWIs can decrease the incidence rates of some early complications and shorten hospitalization time, but causes no significant adverse effects.
2012 Vol. 14 (07): 502-505 [Abstract] ( 5295 ) [HTML 1KB] [PDF 934KB] ( 1805 )
506 GUO Hong-Xiang, ZHANG Jian-Jiang, SHI Pei-Pei, FU Shu-Qin, ZHANG Li-Guo, WANG Miao, LU Feng-Xia
A clinico-pathological comparison between Henoch-Schonlein purpura nephritis and IgA nephropathy in children
OBJECTIVE: To study the difference in clinico-pathological features between IgA nephropathy (IgAN) and Henoch-Schonlein purpura nephritis (HSPN) in children. METHODS: The medical data of 103 children with HSPN and 61 children with IgAN were retrospectively studied. RESULTS: There were no significant differences in age, sex and disease course between the HSPN and IgAN groups (P>0.05). Clinical classification demonstrated that more severe conditions were found in the IgAN group than in the HSPN group and gross hematuria was more common in the IgAN group (P<0.05). Serum creatinine and cholesterol levels were higher in the IgAN group than in the HSPN group (P<0.05). Fibrinogen-related antigen deposition was more common in the HSPN group, while complement 3(C3) deposition was more common in the IgAN group. Interstitial fibrosis, tubular casts and tubular inflammatory infiltration were also more common in the IgAN group (P<0.05). CONCLUSIONS: Significant clinico-pathological differences can be found between HSPN and IgAN in children, and these differences do not support a one disease entity hypothesis.
2012 Vol. 14 (07): 506-509 [Abstract] ( 5958 ) [HTML 1KB] [PDF 934KB] ( 1710 )
510 HUANG Yong-Lan, LI She-Yong, ZHAO Xiao-Yuan, FAN Li-Ping, LIN Wen-Chun, ZHOU Zhi-Hong, CHENG Jing, LIU Li
Enzymatic diagnosis and clinical characteristics of 52 children with mucopolysaccharidosis
OBJECTIVE: To explore the incidence of various types of mucopolysaccharidosis (MPS) and their clinical characteristics. METHODS: A total of 75 children highly suspected as having MPS underwent quantitative and electrophoretic analysis of urinary glycosaminoglycans (GAGs) and enzymatic analysis of seven types of MPS from January 2009 to December 2011. Fluorescence assay was used to measure the activities of α-L-iduronidase, iduronate-2-sulfatase, α-N-acetylglucosaminidase, galactosamine-6-sulfatase, β-galactosidase, arylsulfatase B and β-glucuronidase in the white blood cells. RESULTS: A total of 52 cases were confirmed with MPS based on clinical, radiological, and enzymatic examinations. The 52 cases, with a mean age of 4.0±2.2 years, included 5 cases of MPS I (10%), 20 cases of MPS II (38%), 20 cases of MPS IVA (38%), 6 cases of MPS VI (12%) and 1 case of MPS VII (2%). No MPS IV B cases or MPS IIIB cases were found. Compared with healthy children of the same age, the GAG/Cr ratio was significantly elevated in 50 confirmed cases of MPS (two MPS IVA cases having no increased ratio). All children with increased urinary GAGs had a confirmed diagnosis of MPS. The age of onset was between 1 and 2 years after birth in most cases, and often complicated by hernia and valvular heart disease. Children with MPS I, MPS II, and MPS VI presented with ugly and unsmooth face, short stature, joint stiffness, and limitation of motion, while children with MPS IVA presented with short stature, skeletal dysplasia, and joint laxity. CONCLUSIONS: Type IVA and type II are the most common in MPS cases, followed by type VI and type I. MPS children are characterized by special appearances including ugly and unsmooth facial appearance, short stature and skeletal dysplasia. Quantitative analysis of urinary GAG, as a simple, rapid, and reliable method, is recommended for screening of MPS.
2012 Vol. 14 (07): 510-514 [Abstract] ( 5314 ) [HTML 1KB] [PDF 1017KB] ( 1540 )
515 FENG Bing-Feng, ZHU Kong-Rong
Serum levels of ferritin and neuron-specific enolase in children with hand-foot-mouth disease complicated by acute viral encephalitis
OBJECTIVE: To study serum ferritin and neuron-specific enolase (NSE) levels in children with hand-foot-mouth disease (HFMD) complicated by acute viral encephalitis and their clinical significance. METHODS: Serum levels of ferritin and NSE were measured using ELISA and electrochemical luminescence in 20 children with HFMD complicated by viral encephalitis (encephalitis group), 20 children with HFMD only (simple HFMD group) and 20 healthy children (control group). RESULTS: Serum levels of ferritin in the encephalitis group (212±71 μg/L) were significantly higher than in the simple HFMD group (85±18 μg/L) and control group (70±15 μg/L) (P<0.01). Serum levels of NSE in the encephalitis group (8.6±2.6 μg/L) were also significantly higher than in the simple HFMD group (6.0±1.3 μg/L) and control group (5.6±1.8 μg/L) (P<0.01). Significantly decreased serum ferritin (126±37 μg/L) and NSE levels (6.8±1.9 μg/L) were found in the encephalitis group (P<0.01) after treatment. CONCLUSIONS: Serum levels of ferritin and NSE in children with HFMD complicated by acute viral encephalitis increase, suggesting that serum ferritin and NSE measurement is useful in the early diagnosis of HFMD complicated by acute viral encephalitis.
2012 Vol. 14 (07): 515-517 [Abstract] ( 4780 ) [HTML 1KB] [PDF 919KB] ( 1180 )
518 ZHENG Zhuo-Jun, TANG Yong-Min
Drug resistance of extended-spectrum-β-lactamases-producing bacteria in children with hematological malignancy after chemotherapy
OBJECTIVE: To study the prevalence and drug resistance of extended-spectrum-β-lactamases (ESBLs)-producing bacteria in blood culture isolated from children with hematological malignancy after chemotherapy. METHODS: Blood samples taken from 3264 children with hematological malignancy and severe infection following chemotherapy between 2002 and 2008 were cultured using the Bact/ALTER 3D blood culture system. VITEK 60 automicroscan was used to identify viral species and to conduct drug resistance tests. The results were indentified according to National Committee for Clinical Laboratory Standard guidelines. RESULTS: Fifty-eight strains of Escherichia coli and fifty-one strains of Klebsiella pneumoniae were isolated. Thirty-eight strains of Escherichia coli and nineteen strains of Klebsiella pneumoniae were ESBLs-producing and these ESBLs-producing strains were less susceptible than those that were non-ESBLs-producing to most antibiotics. Both ESBL- and non-ESBL-producing strains were susceptible to imipenem, piperacillin/tazobactam and amikacin. CONCLUSIONS: The prevalence of ESBLs-producing bacteria is high in childrn with hematological malignancy and infection following chemotherapy. ESBLs-producing bacteria are resistant to common antibiotics, suggesting that antibiotic treatment based on the result of antimicrobial susceptibility test is necessary in these children.
2012 Vol. 14 (07): 518-520 [Abstract] ( 4295 ) [HTML 1KB] [PDF 921KB] ( 1189 )
521 DONG Wei, SHENG Jun, GU Xiu-Ming, YAN Hua-Jie, ZENG Chun-Yun, XU Hui-Ting, QIAN Dan, HUANG Qiu-Lan, SHAO Jie
Allergens and their relationships to childhood bronchial asthma in the Jiading District of Shanghai
OBJECTIVE: To study allergens and their relationship to the occurrence of childhood bronchial asthma in the Jiading District of Shanghai. METHODS: Three hundred and eighty-two 4 to 12-year-old children with asthma in the remission stage from Nanxiang Hospital in the Jiading District of Shanghai were used as a case group (asthma group), and 402 children from two primary schools and two kindergartens in Jiading were enrolled by cluster sampling and served as control group. Parents of the children completed a questionnaire on living conditions and allergy-related disease history. Skin prick test (SPT) for 18 common allergens was carried out in both groups. In order to examine the effect of environment and living conditions on SPT results, children in the control group were further divided into two sub-groups according to birth place: migrant (219 cases) and resident (183 cases). RESULTS: SPT results revealed that the main allergens identified in the Jiading region were dermatophagoides farinae, house dust mites, shrimps, cockroaches, and dog hair. The SPT positive rate was 67.9% in the asthma group, and this was significantly higher than in the control group (31.8%) (P<0.01). The environment and living conditions in the migrant group were significantly different from the resident group (P<0.01), whereas the SPT positive rate for this group was significantly lower than in the resident group (P<0.01). CONCLUSIONS: Allergens in the Jiading region mainly originate from dermatophagoides farinae, household dust mites, shrimps, cockroaches and dog hair. Children with asthma are more susceptible to allergens. Environment and living conditions may be relevant, to a certain extent, to an SPT positive rate.
2012 Vol. 14 (07): 521-523 [Abstract] ( 4522 ) [HTML 1KB] [PDF 981KB] ( 1082 )
524 ZHANG Xiao-Ning, YANG Juan, LUO Zheng-Xiu, LUO Jian, REN Luo, LI Bo, CHEN Kun-Hua, FU Zhou, LU Quan, LIU En-Mei
Etiology of nonspecific chronic cough in children and relationship between TRPV1 gene polymorphisms and nonspecific chronic cough
OBJECTIVE: To explore the causes of nonspecific chronic cough in children and relationship between transient receptor potential vanilloid 1 (TRPV1) gene polymorphisms and nonspecific chronic cough. METHODS: A total of 195 children with chronic cough were followed up half a month, one month and three months after their first visit to hospital. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to examine polymorphisms of the TRPV1 gene in the children. A total of 205 healthy or surgical children without chronic cough served as the control group. RESULTS: The etiologic distribution of the 195 children with chronic cough was as follows: 96 (49.2%) cases of cough variant asthma (CVA), 48 (24.6%) cases of CVA complicated by upper airway cough syndrome (UACS), 34 (17.4%) cases of post-infectious cough, and 17 (8.7%) cases of UACS. Three genotypes were identified in both groups at positions rs222747 (CC, GC and GG), rs222748 (CC, TC and TT) and rs8065080 (CC, TC and TT). The frequencies of genotype and allele at position rs222747 did not accord with the law of Hardy-Weinberg. There was no significant difference in frequencies of genotype and allele at positions rs222748 and rs8065080 between the two groups. CONCLUSIONS: CVA, UACS and post-infectious cough are common causes of nonspecific chronic cough in children. TRPV1 gene polymorphisms at positions rs222748 and rs8065080 may be unrelated to nonspecific chronic cough in children.
2012 Vol. 14 (07): 524-528 [Abstract] ( 5270 ) [HTML 1KB] [PDF 1236KB] ( 1273 )
529 HU Chun-Yan, YUE Li-Jie, YU Jie, ZHANG Hong-Hong, YANG Chun-Lan
A novel missense mutation of folypolyglutamate synthetase gene
OBJECTIVE: To examine allelic frequencies of coding single nucleotide polymorphisms (cSNPs) of folypolyglutamate synthetase (FPGS) gene in Chinese Han children with acute leukemia (AL), in order to provide a basis for detecting the relationship between FPGS genetic polymorphisms and tumor individualized chemotherapy. METHODS: cSNPs of exon 5 were detected with polymerase chain reaction (PCR) - denaturing gradient gel electrophoresis (DGGE) in 91 children with AL and 124 children with upper respiratory infection as controls. Genotypes and allelic frequencies were examined. RESULTS: A novel missense mutation, 502/490 T>C (L151/101P), was found in exon 5 of FPGS from control children. The novel mutation was found in mitochondrial variants in two cases and cytosolic variants in three cases. The cytosolic and mitochondrial variants displayed allelic frequencies of 0.70 % and 0.47 % respectively. The novel mutation was not associated with susceptibility to AL. CONCLUSIONS: A novel missense mutation 502/490 T>C (L151/101P) in exon 5 of FPGS gene is firstly found in Chinese Han children, and the cytosolic and mitochondrial variants display allelic frequencies of 0.70 % and 0.47 % respectively.
2012 Vol. 14 (07): 529-532 [Abstract] ( 4094 ) [HTML 1KB] [PDF 1103KB] ( 991 )
533 SHI Yan-Rong, LIU Xiao-Qing, DAI Hong, LU Dian-Yuan, NI Jun-Yi, LI Xi-Hua
The value of high-frequency ultrasound in the diagnosis of duchenne muscular dystrophy in children
OBJECTIVE: To study the value of high-frequency ultrasound in the diagnosis of duchenne muscular dystrophy diseases (DMD) in children. METHODS: Eight children with DMD were enrolled as DMD group and 10 healthy children as the control group. The echogenicity of the rectus femoris muscle and the gap between the gastrocnemius and soleus muscles in the two groups were detected by high-frequency ultrasound. RESULTS: Compared with the control group, rectus femoris and gastrocnemius muscles in the DMD group showed increased echogenicity and their muscle fibers were arranged irregularly, and the gap between the gastrocnemius and soleus muscles became wilder (P<0.01). CONCLUSIONS: High-frequency ultrasound is valuable in the diagnosis of DMD.
2012 Vol. 14 (07): 533-535 [Abstract] ( 3808 ) [HTML 1KB] [PDF 1334KB] ( 1020 )
536 LIN Xiao-Bi, WANG Xiao-Mei, LIU Pei-Ning, ZHANG Ying, WANG Yi-Wen, CHEN Ke, YE Yi, CHEN Yi-Li, ZHOU Yong-Hai
Analysis of hepatitis B surface antibody in 3022 children
OBJECTIVE: To investigate the positive rate of hepatitis B surface antibody (HBsAb) in children. METHODS: Blood samples from 3022 children who received a physical examination in outpatient departments from 2009 to 2011 were subjected to serological test using ELISA to measure the positive rate of HBsAb. RESULTS: The positive rate of HBsAb decreased with age (P0.05), however the positive rate of HBsAb in boys aged one year and over was lower than in girls of the same age (P<0.01). The positive rate of HBsAb in boys aged between 3 and 4 years was higher than in girls of the same age (P<0.01). The positive rate of HBsAb decreased with age in boys, and was lower in those aged two years and over than in those aged one year and over (P<0.01). The positive rate of HBsAb also decreased with age in girls, with significant differences between different age groups (P<0.01). CONCLUSIONS: The positive rate of HBsAb decreases with age in children, so younger children have a higher risk of infection with hepatitis B virus. Serological monitoring of hepatitis B needs to be enhanced.
2012 Vol. 14 (07): 536-538 [Abstract] ( 4091 ) [HTML 1KB] [PDF 916KB] ( 1116 )
539 ZHANG Ge-Xiang, YU Xiao-Long, MA Jian-Hua, HUANG Cai-Xia, GU Dong-Ying, ZHANG Lan
Changes in the physical growth of children aged 3 to 6 years in urban areas of Lanzhou from 2001 to 2010
OBJECTIVE: To study changes in the physical growth of preschool children aged 3 to 6 yeas in urban areas of Lanzhou from 2001 to 2010. METHODS: Stratified, randomized, cluster sampling was used to collect physical examination data on children from 35 private and public kindergartens located in different urban areas of Lanzhou in 2001, 2006, and 2010. Changes in physical growth were analyzed using body height, body weight and body mass index (BMI) as main indices. Growth retardation, underweight, overweight, emaciation and obesity were screened out using height for age (HAZ), weight for age (WAZ), and weight for height (WHZ) and changes from 2001 to 2010 were analyzed. RESULTS: Body height, body weight and BMI increased from 2001 to 2010 in children at different ages (P<0.05). Body height and weight increased with age, while BMI decreased with age. Mean values of HAZ, WAZ, and WHZ increased over time, showing that prevalence rates of underweight, growth retardation, and emaciation decreased from 2001 to 2010 while those of overweight and obesity increased. CONCLUSIONS: Changes in the physical growth of preschool children in urban areas of Lanzhou from 2001 to 2010 were obvious, with increases in body height and body weight. However, problems such as overweight and obesity emerged. In response, while malnutrition is being solved, attention should be paid to over-nutrition that has an adverse effect on physical growth.
2012 Vol. 14 (07): 539-542 [Abstract] ( 4514 ) [HTML 1KB] [PDF 924KB] ( 1021 )
EXPERIMENTAL RESEARCH
543 ZHENG Rui-Dan, WANG Wu-Jin, YING Yan-Qin, LUO Xiao-Ping
Effects of intrauterine growth retardation with catch-up growth on sugar-lipid metabolism and adipocyte function in young rats
OBJECTIVE: To study changes of glycolipid metabolism and adipocyte function in an catch-up growth intrauterine growth retardation (IUGR) rat model. METHODS: IUGR rat model was established by maternal nutrition restriction during pregnancy. Newborn IUGR pups were used as IUGR group, and normal newborn pups were used as control group (appropriate for gestational age, AGA group). At age of 12 weeks, plasma samples were collected for the test of triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), adiponectin and acylation stimulating protein (ASP). Oral glucose tolerance test (OGTT) was performed for the test of glucose and insulin levels, and insulin resistance index (IRI) was calculated. Expression of glucose transfer 4 (GLUT-4) in adipocytes was examined by confocal microscopy. RESULTS: Body weight and BMI in the IUGR group were significantly higher than in the AGA group by 12 weeks (P<0.01), and plasma TC, TG and LDL-C levels in the IUGR group were higher than in the AGA group, but HDL-C was lower (P<0.05). In the OGTT test, blood glucose level and IRI score in the IUGR group were higher than in the AGA group (P<0.05). Compared with the AGA group, the IUGR group had a higher ASP level (P<0.05) and a lower adiponection level (P<0.05). GLUT4 expression in the adipocytes was significantly lower in the IUGR group than in the AGA group (P<0.05). CONCLUSIONS: Catch-up growth may be obviously noted in IUGR rats after birth. Both hyperlipidaemia and insulin resistance occur at age of 12 weeks. Dysfunction of adipocytes decreased expression of GLUT-4 may be risk factors for insulin resistance in IUGR rats.
2012 Vol. 14 (07): 543-547 [Abstract] ( 5388 ) [HTML 1KB] [PDF 970KB] ( 1625 )
548 LI Wen-Juan, CHEN Hui-Jin, QIAN Long-Hua, MAO Feng-Xia
Endogenous self-repair in immature white matter induced by ischemia in neonatal rats Hot!
OBJECTIVE: To study in vivo the endogenous self-repair mechanism in immature white matter induced by ischemia in neonatal rats with periventricular leukomalacia (PVL). METHODS: Five-day-old neonatal Sprague-Dawley (SD) rats were randomly divided into sham and PVL groups. Rat model of PVL was prepared by ligation of the right common carotid artery following 2 hours of exposure to 8% oxygen. Pathological changes and myelination in the white matter were assessed under light and electron microscopy at 7 and 21 days after PVL. O4-positive OL precursor cells in the white matter were determined with immunofluorescence staining. Activation, proliferation, migration and differentiation of glial progenitor cells in SVZ were observed using immunofluorescent double labeling of either NG2 (marker of progenitor cells) and 5-bromodeoxyuridine (BrdU), or O4 (marker of OL precursor cells) and BrdU. RESULTS: All rats in the PVL group manifested either mild or severe white matter injury under light microscopy, and had higher pathological scores of white matter compared with the sham group at 7 and 21 days after PVL (P<0.05). Electron microscopy showed that the number and thickness of myelin sheath in the PVL group were significantly reduced compared with the sham group (P<0.01). O4-positive OL precursor cells in the white matter observed under fluorescence microscopy were significantly reduced in the PVL group compared with the sham group (P<0.05). BrdU/NG2-positive cells in the SVZ increased significantly in the PVL group 48 hours after PVL and migrated into the periventricular area, reaching a peak on day 7 after PVL. BrdU/O4-positive newborn cells began to appear in the periventricular area 72 hours after PVL, and the number of BrdU/O4-positive cells in the PVL group was statistically more than in the sham group on day 21 after PVL (P<0.05). CONCLUSIONS: Ischemia may induce brain self-repair in neonatal rats, resulting in activation and proliferation of NG2 glial progenitor cells in the SVZ migration and differentiation into OL precursor cells in periventricular white matter.
2012 Vol. 14 (07): 548-553 [Abstract] ( 4534 ) [HTML 1KB] [PDF 2298KB] ( 1039 )
CLINICAL EXPERIENCE
554 JIA Rui, YANG Xiao-Feng, LU Yan, LU Nai-Wu, LIU Fu-Qing, ZHANG Yi-Bin, WANG Hong-Mei, ZHOU Jin-Xu, LIU Xin-Ping, LU Xin, LIN Ning
Treatment of hemorrhagic cystitis following stem cell transplantation with umbilical cord-mesenchymal stem cells in 8 children
No abatract available
2012 Vol. 14 (07): 554-557 [Abstract] ( 3746 ) [HTML 1KB] [PDF 924KB] ( 1220 )
CASE REPORT
558 LU Shan, XING Yan, WEI Ling, CHANG Yan-Mei, WANG Xue-Mei
Fatal case in an infant revealing atypical Kawasaki disease
No abstract available
2012 Vol. 14 (07): 558-559 [Abstract] ( 3323 ) [HTML 1KB] [PDF 896KB] ( 1062 )
560 HAN Tong-Yan, PIAO Mei-Hua, GE Ying-Nian, WEI Hong-Ling
Ventilator-associated pneumonia caused by multi-drug resistant Acinetobacter baumannii infection in a extremely low birth weight infant
No abstract avaibale
2012 Vol. 14 (07): 560-561 [Abstract] ( 3532 ) [HTML 1KB] [PDF 886KB] ( 982 )
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