Abstract Objective To study the changes of Hes-1, the target gene of Notch signaling pathway, and its relationship with airway inflammation and remodeling in a rat model of asthma. Methods Forty-eight rats were randomly divided into an asthma group and a control group. The rats in the asthma group were sensitized and challenged by ovalbumin (OVA), and normal saline was used in the control group. Two groups were further divided into 3 subgroups according to time points after challenging, i.e. 4 weeks, 8 weeks and 12 weeks (n=8 rats each). Pathological changes of lungs were observed by light microscopy and the thickness of bronchial smooth muscle layer (Wam) was measured. The levels of IL-4 and INF-γ in rat serum and bronchoalveolar lavage fluids (BALF) were measured using ELISA. Expression levels of Hes-1 protein and mRNA were determined by immunohistochemistry and quantitative real-time PCR respectively. Results Together with the extension of challenging, the Wam of rats in the asthma group increased, a decrease of INF-γ level and an increase of IL-4 level in serum and BALF were also observed, and the differences were statistically significant compared with those in the corresponding control group (PPPConclusions Levels of Hes-1 protein and mRNA increased, which were closely related with the levels of airway inflammatory factors and remodeling of airway smooth muscle. Hes-1 may play an important role in the pathogenesis of asthma.
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Cite this article:
QIANG Ba-Cuo-Zhen,XIE Liang,LIU Han-Min et al. Relationship between Hes-1 and airway inflammation and remodeling in a rat model of asthma[J]. CJCP, 2014, 16(6): 648-653.
QIANG Ba-Cuo-Zhen,XIE Liang,LIU Han-Min et al. Relationship between Hes-1 and airway inflammation and remodeling in a rat model of asthma[J]. CJCP, 2014, 16(6): 648-653.
Kudo M, Ishigatsubo Y, Aoki I. Pathology of asthma[J].Front Microbiol, 2013, 4: 263.
[5]
Krishnamoorthy N1, Oriss TB, Paglia M, et al. Activation of c-Kit in dendritic cells regulates T helper cell differentiation and allergic asthma[J].Nat Med, 2008, 14(5): 565-573.
[6]
Kang JH, Kim BS, Uhm TG, et al. Gamma-secretase inhibitor reduces allergic pulmonary inflammation by modulating Th1 and Th2 responses[J].Am J Respir Crit Care Med, 2009, 179(10): 875-882.
