CJCP
  中文版
  English Version
 
 
2011 Vol.  13 No.  4
Published: 2011-04-15
CLINICAL RESEARCH
EXPERIMENTAL RESEARCH
CLINICAL EXPERIENCE
CASE REPORT
REVIEW
CONTINUING EDUCATION
CLINICAL RESEARCH
273 HUANG Dan-Lin, XU Zi-Chuan, DANG Xi-Qiang, ZENG Xue-Qi, HE Xiao-Jie, YI Zhu-Wen, HE Qing-Nan
Relationship between renal Th1/Th2 ratio and renal microvascular injury in children with Henoch-Schonlein purpura nephritis
OBJECTIVE: To explore possible correlations between renal Th1/Th2 ratio and renal microvascular injury in children with Henoch-Schonlein purpura nephritis (HSPN). METHODS: Thirty-two children with HSPN were enrolled. They were classified into four groups by renal pathology: HSPN class II (n=8), HSPN class IIIa (n=7), HSPN class IIIb (n=10) and HSPN class IV/V (n=7). Five patients undergoing nephrectomy due to trauma were used as the controls. INFγ, IL-4 and CD34 in the renal tissues were measured by immunohistochemical analysis. INFγ was used as a marker of Th1, IL-4 was used as a marker of Th2 and CD34 was used as a marker of microvessel. The renal microvessel density was evaluated according to the Weidner standard. The relationships among the local Th1/Th2 ratio, renal pathological grade, microvessel score and microvessel density were studied. RESULTS: Immunohistochemical analysis showed a lower expression of INFγ and a higher expression of IL-4 in the HSPN groups than in the control group. The local Th1/Th2 ratio in the HSPN groups decreased and correlated significantly with the renal pathological grade. There were significant differences among four HSPN subgroups (P<0.05). Compared with the control group, the renal microvessel density in the HSPN class II and class IIIa groups increased significantly (P<0.05), but it decreased in the HSPN class IV/V group (P<0.05). The renal microvessel scores in the HSPN class IIIa, class IIIb and class IV/V groups increased significantly compared with those in the control and the HSPN classⅡ. The increased renal microvessel scores were associated with more severe renal pathological changes. A negative correlation was found between the local Th1/Th2 ratio and the microvessel density in kidneys (r=-0.921, P<0.01). CONCLUSIONS: The decrease of Th1/Th2 ratio in kidneys might be responsible for renal microvascular injury in children with HSPN.
2011 Vol. 13 (4): 273-277 [Abstract] ( 7063 ) [HTML 1KB] [PDF 1698KB] ( 2115 )
278 LI Jing, WANG Yi-Bing, LIU Hua-Lin, JIANG Yu-Hong, LIU Wei, WANG Ya-Qiu
P-selectin gene -2123 polymorphism in children with Henoch-Schonlein purpura
OBJECTIVE: To investigate whether P-selectin gene -2123 polymorphism is associated with the pathogenesis of Henoch-Schonlein purpura (HSP) in children. METHODS: Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) is used to identify the distribution of allele and genotype frequencies of P-selectin gene promoter -2123 polymorphism in 86 children with HSP (including 40 cases of purpura nephritis) and 70 healthy controls. RESULTS: Compared with the healthy controls, the frequencies of GG genotype and G allele of P-selectin promoter -2123 in children with HSP increased significantly (P<0.05). There were no significant differences in P-selectin promoter -2123 genotype and allele frequencies between the patients with and without nephritis. CONCLUSIONS: P-selectin gene promoter -2123 polymorphism appears to be associated with the pathogenesis of HSP in children.
2011 Vol. 13 (4): 278-281 [Abstract] ( 6837 ) [HTML 1KB] [PDF 967KB] ( 1521 )
282 WANG Xue-Ping, QIU Yun-Shu, HAO Guo-Ping, ZHU Lei
Levels of regulatory T cells in peripheral blood of children with idiopathic thrombocytopenic purpura
OBJECTIVE: To study the levels of CD4+CD25+CD127- and CD3+CD4-CD8- regulatory T (Treg) cells in peripheral blood of children with idiopathic thrombocytopenic purpura (ITP). METHODS: The flow cytometry was used to detect the expression of CD4+CD25+CD127- and CD3+CD4-CD8- Treg cells in peripheral blood of 33 children with ITP and 21 healthy children. RESULTS: The expression levels of CD4+CD25+CD127-［(2.7±1.7)% vs (4.8±1.6)%; P<0.01］ and CD3+CD4-CD8-［(5.2±3.1)% vs (8.1±3.5)%; P<0.01］Treg cells in children with ITP were significantly lower than in the controls. CONCLUSIONS: The expression levels of CD4+CD25+CD127- and CD3+CD4-CD8- Treg cells decrease in children with ITP, suggesting that CD4+CD25+CD127- and CD3+CD4-CD8- Treg cells might play a role in the pathogenesis of ITP.
2011 Vol. 13 (4): 282-284 [Abstract] ( 6265 ) [HTML 1KB] [PDF 925KB] ( 1889 )
285 SUN Dan, LIU Zhi-Sheng, YAO Hui, KANG Shi-Xiu, HE Cai-Ying, HU Jia-Sheng, WU Ge-Fei, WANG Fang-Lin
Effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells of children with febrile seizures
OBJECTIVE: To study the effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells (PBMC) of children with febrile seizures (FS). METHODS: Sixteen children with FS and 16 age- and gender-matched healthy children were enrolled. The samples of PBMC from FS children were randomized into two groups with or without TNF-α treatment (TNF-α concentration 1.0 ng/mL). PBMC were purified and cultured with a conventional method in vitro. The expression of ICAM-1 and LFA-1 in PBMC was determined by flow cytometry (FCM). RESULTS: ICAM-1［(20±9)% vs (14±7)%)］and LFA-1［(43±16)% vs (30±16)%］expression in PBMC in the untreated FS group was significantly higher than that in the normal control group (P<0.05). Compared with the untreated FS group, the treatment with TNF-α remarkably increased the ICAM-1 expression［(27±11)%］(P<0.05). PBMC LFA-1 expression［(52±21)%］in the TNF-α-treated group was higher than that in the untreated FS group, although there were no statistical differences between the two groups. CONCLUSIONS: TNF-α treatment may increase LFA-1 and ICAM-1 expression in PBMC of children with FS.
2011 Vol. 13 (4): 285-287 [Abstract] ( 6903 ) [HTML 1KB] [PDF 930KB] ( 1590 )
288 LI Xi-Hua, ZHAO Lei, WU Yan, WU Jie, HE Da-Ke, LIU Xiao-Qing
Diagnostic value of neuronal nitric oxide synthase antibody for clinically suspected Becker muscular dystrophy Hot!
OBJECTIVE: Immunohistochemistry using antibodies to dystrophin is the pathological basis for the differential diagnosis of Duchenne and Becker muscular dystrophy (DMD and BMD). In rare cases, however, labelling dystrophin on sarcolemma is equivocal and similar to that observed in controls. This makes the diagnosis of BMD difficult. This study aimed to explore the diagnostic value of neuronal nitric oxide synthase (nNOS) antibody for clinically suspected BMD. METHODS: Immunohistochemical staining was performed on muscle specimens of 5 cases of BMD with positive expression of Dys-C (3 cases had a confirmed diagnosis of BMD, 2 cases were clinically suspected as BMD) by using dystrophin and nNOS antibodies. Normal muscle specimens from the children with fracture were used as controls. RESULTS: Compared with the controls, the expression of Dys-R, Dys-C and Dys-N was markedly reduced and nNOS was not expressed on sarcolemma in the three cases of definitely diagnosed BMD. The two cases of clinically suspected as BMD had a complete absence of sarcolemmal nNOS, even if had a similar expression of dystrophin on sarcolemma to the controls. CONCLUSIONS: nNOS antibody staining can be used for a definite diagnosis in children with clinically suspected BMD who have the almost normal expression of dystrophin.
2011 Vol. 13 (4): 288-291 [Abstract] ( 7225 ) [HTML 1KB] [PDF 1485KB] ( 1504 )
292 WANG Xi-Ge, CAO Yi-Ming, WANG Xiao-Ge
Detection and significance of CD4+ CD25int/high CD127low regulatory T cells in pediatric aplastic anemia
OBJECTIVE: To study the expression of CD4+ CD25int/high CD127low regulatory T cells in peripheral blood (PB) and its relation to the quantity of Hb, WBC and platelet (Plt) in children with aplastic anemia (AA). METHODS: Expression of CD4+ CD25int/high CD127low in PB was detected by flow cytometry in 22 children with AA before and after treatment and in 15 healthy controls. The relationships between CD4+CD25highCD127low and the quantity of Hb, WBC and Plt were evaluated. RESULTS: Compared to controls, the percentages of CD4+ CD25+/CD4+, CD4+CD25high/CD4+, CD4+ CD25+ CD127low/CD4+ and CD4+CD25highCD127low/CD4+ in PB of AA patients decreased markedly at the active phase (P﹤0.05) . By the recovery phase, the percentages of CD4+CD25+/CD4+, CD4+CD25high/CD4+, CD4+ CD25+ CD127low/CD4+ and CD4+CD25highCD127low/CD4+ increased significantly to the levels similar to the controls. There were significant positive relationships between the expression of CD4+CD25highCD127low cells and the quantity of Hb, WBC and Plt (r=0.499, 0.526, 0.540 respectively; P﹤0.05). CONCLUSIONS: The decrease of the percentage of CD4+CD25int/highCD127low regulatory T cells might be associated with the development of pediatric AA. The CD4+CD25int/highCD127low regulatory T cells can serve as a marker for the evaluation of disease severity as well as a target of further study on immune treatment of AA.
2011 Vol. 13 (4): 292-295 [Abstract] ( 7202 ) [HTML 1KB] [PDF 943KB] ( 1582 )
296 LOU Yan, YIN Na, CHEN Feng-Qin, CHENG Ya-Ying, XU Li-Jin, DAI Fang, SONG Xiao-Tao
Selective screening of inborn errors of metabolism by using the tandem mass spectrometry: pilot study of 552 children at high risk
OBJECTIVE: To study the application of tandem mass spectrometry (MS/MS) in the selective screening of inborn errors of metabolism (IEM) in high risk children and to understand the positive rate and types of IEM. METHODS: MS/MS was used to examine 552 blood samples from high risk cases of IEM who came from 8 hospitals in Shijiazhuang, Hebei Province. RESULTS: Sixty-four children (11.6%) were confirmed with IEM by the MS/MS, including 33 cases of methylmalonic acidemia or propionic acidemias, 2 cases of phenylketonuria, 3 cases of carnitine palmotoyl transferase I deficiency, 1 case of long-chain acyl-CoA dehydrogenase deficiency, 2 cases of medium-chain acyl-CoA dehydrogenase deficiency, 6 cases of maple syrup urine disease, 2 cases of short-chain acyl-CoA dehydrogenase deficiency, 2 cases of glutaric acidemia type I, 2 cases of isovaleric acidemia, 2 cases of homocystinuria, 4 cases of carnitine deficiency, 1 case of tyrosinemia, 1 case of argininosuccinic aciduria, 2 cases of citrullinemia and 1 case of argininemia. CONCLUSIONS: MS/MS can be used to screen and classify IEM.
2011 Vol. 13 (4): 296-299 [Abstract] ( 7582 ) [HTML 1KB] [PDF 932KB] ( 3070 )
300 YAN Hua-Jie, SHENG Jun, DONG Wei, SHAO Jie
Clinical characteristics of human bocavirus infection in 90 children
OBJECTIVE: To study the clinical characteristics of human bocavirus (HBoV) infection in children. METHODS: Nasal and throat swab samples were collected in 843 children with lower respiratory tract infection. The multiple RT-PCR method was used to detect HBoV and six other common respiratory tract viruses. The clinical characteristics of HboV positive cases were investigated. RESULTS: Among 843 cases, 90 were HboV positive (10.7%), 131 were respiratory syncytial virus (RSV) positive (15.5%), 117 were influenza virus positive (13.9%), 84 were parainfluenza virus positive (10.0%), 55 were rhinovirus positive (6.5%), 48 were coronavirus positive (5.7%), and 33 were human metapneumovirus positive (3.7%). Of the 90 HBoV infected patients, 45 (50%) showed a co-infection with other respiratory tract viruses. Among them, 33 were infected with one other type of virus (37%), 11 (12%) were infected with two other types of virus, and 1 case (1%) was infected with other three viruses. The HBoV positive rate in children with wheezing was significantly higher than those without wheezing (17.0% vs 9.2%; P<0.01). The common clinical manifestations of HBoV-infected patients included frequent coughing, wheezing and fever. There were no significant differences in the frequency of wheezing between HBoV and RSV infected patients. CONCLUSIONS: HBoV positive rate detected from children with wheezing is higher than from children without wheezing, suggesting that apart from RSV, HBoV is another virus causing wheezing in children with respiratory tract infection. Co-infections of HBoV with other respiratory track viruses can be present in some patients.
2011 Vol. 13 (4): 300-302 [Abstract] ( 7604 ) [HTML 1KB] [PDF 928KB] ( 1635 )
303 WEN Peng-Qiang, WANG Guo-Bing, CHEN Zhan-Ling, CUI Dong, YUAN Quan, SONG Ping, CHEN Shu-Li, LIAO Jian-Xiang, LI Cheng-Rong
SLC25A13 gene analysis in neonates with intrahepatic cholestasis caused by citrin deficiency
OBJECTIVE: Neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD) which resulted from mutation in SLC25A13 gene can present transient intrahepatic cholestasis, low birth weight, growth retardation, hypoproteinemia and so on. This study aimed to identify the mutation type of NICCD patients by DNA sequencing. METHODS: Twenty children diagnosed as NICCD were consented to enroll in this study. PCR assays were performed to amplify the eighteen exons and its flanking sequences of SLC25A13 gene, which were defined as the upstream and downstream 50 bp from starting and ending site of the exons. Then the PCR products were purified and followed by automated DNA sequencing. The IVS16ins3kb mutation was detected by nested PCR and RT-PCR. RESULTS: Seven genetic variations of SLC25A13, termed as 851del4, 1638ins23, IVS16ins3kb, IVS6+5G>A, c.775C>T (p.Q259X), c.1505C>T (p.P502L) and c.1311C>T (p.C437C), were identified in the subjects, of which c.775C>T (p.Q259X), c.1505C>T (p.P502L) and c.1311C>T (p.C437C) were reported for the first time in NICCD patients. And a compound mutation of ［1638ins23+IVS16ins3kb］ was also identified. In 20 patients with NICCD, 6 patients were 851del4 homozygotes, 7 patients were compound heterozygotes, and 7 patients were heterozygotes of single mutation. 851del4 was the major mutation type (64%), followed by 1638ins23 (15%), IVS16ins3kb (12%) and IVS6+5G>A (6%). CONCLUSIONS: 851del4 is the major mutation type in Chinese patients with NICCD.
2011 Vol. 13 (4): 303-308 [Abstract] ( 9578 ) [HTML 1KB] [PDF 1348KB] ( 3198 )
EXPERIMENTAL RESEARCH
309 WANG Hui-Qing, XIONG Ying, GUO Wen-Jun
Expression of iNOS protein and gliacyte apoptosis in neonatal rats with white matter damage
OBJECTIVE: Inducible nitric oxide synthase (iNOS) is a main rate-limiting enzyme resulting in over-production of nitric oxide following hypoxia-ischemia (HI). The aim of this study was to observe the expression of iNOS protein and gliacyte apoptosis in the brains of premature rats after HI, in order to explore possible relationships of iNOS with white matter damage (WMD). METHODS: One hundred and twelve 2-day-old premature rats were randomly subjected to right carotid ligation followed by 4 hrs hypoxic stress (WMD group) or sham operation (control group). The pups were sacrificed at 1, 3, 6, 12 hrs, and 1, 3 and 7 days after HI. Immunohistochemical technique was applied to determine the iNOS expression in periventricular white matter tissues. Gliacyte apoptosis was detected in these tissues by TUNEL. RESULTS: Compared with the control group, iNOS expression began to increase 1 hr after HI and reached the peak 1 day after HI in the WMD group. Gliacyte apoptosis increased 1 hr after HI and peaked 1 day after HI in the WMD group compared with the control group. CONCLUSIONS: In the neonatal rats with WMD, the expression of iNOS may be involved in the ischemic cellular events including apoptosis, and plays a role in the pathophysiological process of WMD.
2011 Vol. 13 (4): 309-312 [Abstract] ( 6017 ) [HTML 1KB] [PDF 1310KB] ( 1681 )
313 DING Xiao-Fang, ZHONG Li-Li, ZHANG Bing, LI Jia
Protective effects of lidocaine on hyperoxia-exposed type Ⅱalveolar epithelial cells from premature rats
OBJECTIVE: To investigate the effects of lidocaine on apoptosis and proliferation of hyperoxia-exposed type Ⅱalveolar epithelial cells (AECⅡ) from premature rats. METHODS: Primary cultured AEC Ⅱ isolated from premature rats were randomly divided into four groups: air, air+ lidocaine (20 μg/mL), 95%O2/5%CO2, and 95%O2/5%CO2+ lidocaine. The cells in each group were collected 24 hrs after culture in ordinary incubators (37℃,5%CO2). The proliferation and apoptosis of AEC Ⅱ were detected by flow cytometry. Protein levels of proliferating cell nuclear antigen (PCNA) were measured by Western blot. RESULTS: The apoptosis rate of AECⅡincreased, the percentages of G2/M and S phase cells decreased and the protein levels of PCNA decreased significantly in the group exposed to 95%O2/5%CO2 compared with the group exposed to air (P<0.01). Lidocaine treatment decreased apoptosis rate of AECⅡ, increased percentage of G2/M and S phase cells, and increased protein levels of PCNA. CONCLUSIONS: Hyperoxia can increase apoptosis and inhibit proliferation of AECⅡ in premature rats. Lidocaine may have protective effects against the AECⅡ injuries.
2011 Vol. 13 (4): 313-316 [Abstract] ( 5535 ) [HTML 1KB] [PDF 1091KB] ( 1337 )
317 LI Xiao-Li, JIA Tian-Ming, LUAN Bin, LIU Tao, YUAN Yan
Effects of electric stimulation at the cerebellar fastigial nucleus on astrocytes in the hippocampus of neonatal rats with hypoxic-ischemic brain damage
OBJECTIVE: To study the effects of electric stimulation at the cerebellar fastigial nucleus on astrocytes in the hippocampus of neonatal rats with hypoxic-ischemic brain damage (HIBD) and the possible mechanism. METHODS: One hundred and eighty 7-day-old neonatal Sprague-Dawley rats were randomly divided into three groups: sham-operation (control group) and HIBD with and without electric stimulation (n=60 each). The HIBD model of neonatal rats was prepared by the Rice-Vennucci method. Electric stimulation at the cerebellar fastigial nucleus was given 24 hrs after the operation in the electric stimulation group once daily and lasted for 30 minutes each time. The other two groups were not subjected to electric stimulation but captured to fix in corresponding periods. Rats were sacrificed 3, 7, 14 and 21 days after stimulations to observe the glial fibrillary acidic protein (GFAP) expression by immunohistochemisty and the ultrastructural changes of astrocytes in the hippocampus under an electron microscope. RESULTS: Immunohistochemical analysis showed the expression of GFAP in the HIBD groups with and without electric stimulation increased significantly compared with the control group on day 3, reached the peak on day 7, and the increased expression remained till to day 21. The GFAP expression in the electric stimulation group was significantly lower than that in the untreated HIBD group at all time points. Under the electron microscope, the astrocytes in the untreated HIBD group were swollen and the amount of organelles was reduced, while the swelling of astrocytes was alleviated and the organelles remained in integrity in the electric stimulation group. CONCLUSIONS: The electric stimulation at the cerebellar fastigial nucleus can inhibit the excessive proliferation of astrocytes and relieve the structural damage of astrocytes in neonatal rats following HIBD.
2011 Vol. 13 (4): 317-320 [Abstract] ( 6520 ) [HTML 1KB] [PDF 1252KB] ( 1648 )
321 ZOU Li-Ping, WANG Li-Xia, ZHANG Yan, DU Wen-Li
Expression of SDF-1 in lung tissues and intervention of AMD3100 in asthmatic rats
OBJECTIVE: To study the expression of stromal cell derived factor-1(SDF-1) in the airway and to investigate the role of SDF-1 receptor antagonist AMD3100 intervention in rats with asthma. METHODS: Thirty Sprague-Dawley rats were randomly divided into three groups: normal control and asthma with and without AMD3100 intervention. The rat model of asthma was prepared by aerosolized ovalbum (OVA) challenge. The AMD3100 intervention group was administered with AMD3100 of 50 μg 30 minutes before challenge every other day, for 10 times. The characteristic airway inflammation and alterations of airway structures were observed by hemetoxylin and eosin staining. The levels of interleukin 4 and interleukin 5 in whole lung homogenates were measured using ELISA. RT-PCR was used to evaluate the expression of SDF-1 mRNA in the lung. RESULTS: The airway wall thickness in the untreated asthma group was greater than that in the control and the AMD3100 intervention groups (P<0.05). The levels of interleukin 4 and interleukin 5 in whole lung homogenates in the AMD3100 intervention group were lower than those in the untreated asthma group (P<0.05). The expression of SDF-1 mRNA in the untreated asthma group was higher than that in the control and the AMD3100 intervention groups (P<0.05). CONCLUSIONS: SDF-1 may be associated with airway inflammation and remodeling in rats with asthma. AMD3100 may reduce the airway inflammation and improve airway remodeling by inhibiting the bioactivity of SDF-1.
2011 Vol. 13 (4): 321-325 [Abstract] ( 6256 ) [HTML 1KB] [PDF 1367KB] ( 1822 )
326 GU Hai-Yan, ZHAO De-Yu, WANG Quan
Effect of maternal vitamin D deficiency on lung morphogenesis and platelet-derived growth factor-A expression in rat offspring
OBJECTIVE: To study the effect of maternal vitamin D deficiency on lung morphogenesis and platelet-derived growth factor-A (PDGF-A) expression in rat offspring. METHODS: Sprague-Dawley (SD) female rats were randomly divided into two groups: normal control and vitamin D deficiency, with 6 rats in each group. The vitamin D deficiecy group was kept away from light and fed with the forage without vitamin D. After 2 weeks, the rats were mated with normal SD male rats. The morphological changes of fetal rat lungs on day 20 of gestation and 1-day-old neonatal rat lungs were observed by light microscope and electronic microscope. The levels of PDGF-A mRNA and protein in fetal and neonatal rat lungs were measured by reverse transcriptase-polymerase chain reaction (RT-PCR) technique and Western blot method respectively. RESULTS: Under the light microscope, smaller alveolar space, smaller diameter of the respiratory membrane and thicker alveolus mesenchyma were observed in lungs of fetal and neonatal rats from the vitamin D deficiency group compared with the controls (P<0.05). Under the electronic microscope, fewer lamellar bodies but more glycogen deposition in intracytoplasm were observed in the lungs of fetal rats from the vitamin D deficiency group compared with the controls. There was an increased number of empty lamellar bodies in neonatal rats from the vitamin D deficiency group. The levels of PDGF-A mRNA and protein in lungs of fetal and neonatal rats from the vitamin D deficiency group were significantly lower than the controls (P<0.05). CONCLUSIONS: Maternal vitamin D deficiency during pregnancy may inhibit the development of lung morphogenesis and PDGF-A expression in late fetal and neonatal rats. The low expression of PDGF-A may be involved in the inhibitory effect of vitamin D deficiency on the lung development.
2011 Vol. 13 (4): 326-330 [Abstract] ( 6425 ) [HTML 1KB] [PDF 1252KB] ( 1655 )
331 JIN Long-Teng, ZHANG He, LI Chang-Chong, ZHANG Wei-Xi, LIN Li
Effects of lentinan on interleukin-1β-induced transdifferentiation of human embryonic lung fibroblasts to myofibroblasts
OBJECTIVE: To study the effects of interleukin-1β (IL-1β) on transdifferentiation of human embryonic lung fibroblasts to myofibroblasts and the effects of lentinan on the transdifferentiation. METHODS: The human embryonic lung fibroblasts were cultured in vitro, and fibroblasts were treated with different concentrations of IL-1β and lentinan. The proliferation activity of the human embryonic lung fibroblasts was evaluated by the Cell Counting Kit-8 (CCK-8). The expression of α-smooth muscle actin (α-SMA) protein was measured by immunocytochemistry. The levels of fibronectin (FN), typeⅠcollagen (ColⅠ) and α-SMA mRNA were detected by RT-PCR. RESULTS: Compared with the untreated control group, the absorbance value of cell proliferation, α-SMA protein levels, FN, ColⅠand α-SMA mRNA expression were significantly up-regulated after different concentrations of IL-1β (0.1, 1, 10 ng/mL) treatment for 48 hrs (P<0.01). Lentinan treatment inhibited up-regulation of the cell proliferation activity, α-SMA protein levels, FN, ColⅠand α-SMA mRNA expression induced by IL-1β in a dose-independent manner (P<0.01). CONCLUSIONS: Lentinan can suppress human embryonic lung fibroblast proliferation, fibroblast-myofibroblast transdifferentiation and extra cellular matrix synthesis induced by IL-1β.
2011 Vol. 13 (4): 331-335 [Abstract] ( 5703 ) [HTML 1KB] [PDF 1367KB] ( 2009 )
336 SHU Lin-Hua, SHANG Yun-Xiao, ZHANG Fu-Hui, ZHANG Han, MA Xiao-Xue, YANG Nan, HUANG Wan-Jie
Effects of Streptococcus pneumoniae on the ultrastructure of alveolar epithelial cells type Ⅱ in the lung tissues of mice and children
OBJECTIVE: To explore the possible mechanisms of lung necrosis by examining the effects of Streptoccus pneumoniae (S.p) on the ultrastructure of alveolar epithelial cells type Ⅱ(AEC-Ⅱ) in the lung tissues of mice and children. METHODS: The suspended solutions of S.p strains cultured from the blood of a child with pneumococcal necrotizing pneumonia (PNP) (0.3 mL, CFU: 1×108/L) were instilled into the trachea of pathogen-free mice to prepare PNP model. The same amount of normal saline was given for the control group (10 mice). The samples (1 mm3) from the lower lobe of right lung of the mice were obtained 92 hrs later and fixed in 2.5% glutaraldehyde. Normal and abnormal lung tissues (1 mm3) were obtained while operation for the left lower lobe pulmonary cavity excision in the child with PNP. The specimens were fixed in 2.5% glutaraldehyde and stored at 4℃. A transmission electron microscope was employed for the examination of the ultrastructure of AEC-Ⅱ in the lung tissues. RESULTS: Quantitative reduction and exfoliation of microvilli in S.p-infected AEC-Ⅱ were observed in both mice and this child compared with the control. Enlarged size, enhanced evacuation and reduced density of the lamellar bodies were also presented. The number of mitochondria was obviously reduced. The nucleolus chromatin concentrated and showed an inhomogeneous distribution. CONCLUSIONS: S.p infection results in comparable damage to the ultrastructure of AEC-Ⅱ in mice and children that may represent one of the primary causes responsible for S.p-induced lung tissue necrosis.
2011 Vol. 13 (4): 336-339 [Abstract] ( 6882 ) [HTML 1KB] [PDF 1170KB] ( 1623 )
340 LI Qiao-Jun, ZOU Li-Ping, WANG Jian-Wen
Signal transduction pathway mediated by thrombopoietin in the inflammation model of microglia
OBJECTIVE: To explore the signal transduction pathway mediated by thrombopoietin (TPO) in the inflammation model of microglia induced by lipopolysaccharide (LPS). METHODS: The inflammation model of microglia BV2 cells was prepared by LPS of 0.5 and 1.0 μg/mL stimulation. The expression of TPO and ERK mRNA in BV2 cells was detected by real time quantitative PCR. Western blot was used to evaluate the expression of TPO and ERK protein in BV2 cells. TPO and IL-6 contents in the culture supernatant fluid were measured using ELISA. RESULTS: LPS stimulation increased significantly the mRNA and protein expression of TPO and ERK in BV2 cells, especially at the concentration of 1.0 μg/mL for 12 hrs stimulation. There was a significant positive correlation between the mRNA and protein expression of TPO and ERK. CONCLUSIONS: Signal transduction pathway of ERK1/2 participates in the activation of TPO in inflammatory injury of BV2 cells.
2011 Vol. 13 (4): 340-343 [Abstract] ( 6107 ) [HTML 1KB] [PDF 1063KB] ( 1908 )
CLINICAL EXPERIENCE
344 SHU Xiao-Mei, ZHANG Gui-Ping, YANG Bing-Zhu, LI Lei
Clinical analysis of 4 cases of childhood Rolandic epilepsy with electrical status epilepticus during sleep
No abstract available
2011 Vol. 13 (4): 344-345 [Abstract] ( 5276 ) [HTML 1KB] [PDF 909KB] ( 1442 )
346 ZHANG Hao-Ye, LIU Zhen-Guo, ZHAO Ling-Ling
Clinical value of 45 cases of childhood influenza A H1N1
No abstract available
2011 Vol. 13 (4): 346-347 [Abstract] ( 3833 ) [HTML 1KB] [PDF 900KB] ( 1286 )
348 WANG Juan-Mei, LI Yun, GUO Chun, FANG Yi-Bing
Clinical value of peak flow meter in measurement of peak expiratory flow in children with asthma
No abstract available
2011 Vol. 13 (4): 348-350 [Abstract] ( 5246 ) [HTML 1KB] [PDF 1018KB] ( 2095 )
CONTINUING EDUCATION
351 LIU Ling, LIU Jian-Ping, CHEN Chao, CAO Yun, Shoo LEE
Information of clinical fellow training in the NICU of the Affiliated Hospital of Ottawa University and the Eastern Ontario Children's Hospital of Canada
No abstract available
2011 Vol. 13 (4): 351-353 [Abstract] ( 4783 ) [HTML 1KB] [PDF 908KB] ( 1946 )
CASE REPORT
354 LU Hua-Jun, ZHAO Zhong-Yan
A case report of alpha 1-antitrypsin deficiency
No abstract available
2011 Vol. 13 (4): 354-355 [Abstract] ( 5210 ) [HTML 1KB] [PDF 908KB] ( 1553 )
356 WANG Xia, SHEN Jia, WU Wei-Lan, WEI Min-Jiang
A case report of Kimura disease
No abstract available
2011 Vol. 13 (4): 356-357 [Abstract] ( 4859 ) [HTML 1KB] [PDF 1056KB] ( 1654 )
REVIEW
358 ZHANG Yong-Ming, LIU Xiu-Yun
Advance in the diagnosis and treatment of Mycoplasma pneumoniae pneumonia and related complications
No abstract available
2011 Vol. 13 (4): 358-360 [Abstract] ( 6481 ) [HTML 1KB] [PDF 923KB] ( 2875 )
CJCP
About Journal
Editors members
Contact Us

 
Copyright © 2004-2005 Chinese Journal Of Contemporary Pediatrics All rights reserved.
Addr: 87 Xiangya Road, Changsha, Hunan, 410008, PRC
Tel: 0731-84327402 Fax: 0731-84327922 E-mail: cjcp1999@csu.edu.cn